麦芽醇提物对肠道微生物区系中大肠埃希菌和金黄色葡萄球菌的体外抑菌机制
In vitro antibacterial mechanism of alcoholic extract of malt against Escherichia coli and Staphylococcus Aureus in intestinal microflora
陶梦娟 1张莉 2武华军 3金文平 3巴红平 4王彬 2陈永刚5
作者信息
- 1. 武汉科技大学医学部,武汉 430065
- 2. 湖北省食品质量安全监督检验研究院,武汉 430075
- 3. 武汉市第三医院药学部,武汉 430060
- 4. 武汉市临床检验中心检验科,武汉 430030
- 5. 武汉科技大学医学部,武汉 430065;武汉市临床检验中心检验科,武汉 430030
- 折叠
摘要
目的 探讨麦芽醇提物对肠道微生物的抗菌活性及体外的抑菌机制.方法 微量肉汤稀释法测定各微生物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),在不同时间点绘制麦芽醇提物处理前后大肠埃希菌和金黄色葡萄球菌的生长曲线.通过荧光显微镜、结晶紫染色实验以及乳酸脱氢酶活性、碱性磷酸酶含量变化来探究其作用机制.结果 麦芽醇提物对选取的8种肠道微生物抑菌效果明显,其中对大肠埃希菌与金黄色葡萄球菌MBC均为25,MIC均为12.5 mg·mL-1.经麦芽醇提物作用后,与对照组相比,大肠埃希菌和金黄色葡萄球菌生物膜形成能力减弱,且呈剂量依赖关系;随着麦芽醇提物浓度的增加,在细菌暴露于2MIC的条件下,其细胞膜的破损程度相较于对照组比较严重;细菌乳酸脱氢酶活性也随着浓度的增加逐渐下降,与对照组相比差异有统计学意义;大肠埃希菌与金黄色葡萄球菌的碱性磷酸酶分别在3 h、6 h时活性最高,随之趋于平稳.结论 麦芽醇提物对肠道微生物具有显著的抗菌活性,主要通过破坏细胞膜的完整性导致胞内物质外泄造成细菌死亡.
Abstract
Objective To determine the antibacteria activity of alcoholic extract of malt on intestinal microorganisms and elucidate its antibacteria mechanism in vitro.Methods The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of each microorganism were determined with the microbroth dilution method.The growth curves of Escherichia coli and Staphylococcus aureus were plotted before and after the treatment with alcoholic extract of malt at various time points.The mechanism of action was measured by fluorescence microscopy,crystal violet staining experiments,lactate dehydrogenase activity assays,and alkaline phosphatase content analyses.Results Maltol extract showed significant antibacterial effect against 8 intestinal microorganisms,with MIC and MBC against Escherichia coli and Staphylococcus aureusall all measured at 25 and 12.5 mg·mL-1,respectively.Biofilm formation ability of Escherichia coli and Staphylococcus aureus was dose-dependently weakened ad compared with that of the control group.At 2MIC,the cell membrane damage in the bacteria treated with alcoholic extract of malt,was very severe compared with that of the control group.Additionally,bacterial lactate dehydrogenase activity decreased gradually with increasing alcoholic extract of malt concentration,obviously different with that of the control group.Alkaline phosphatase content peaked at 3 hours for Escherichia coli and 6 hours for Staphylococcus aureus before the stabilizing.Conclusion Maltol extract demonstrates great antibacterial activity against intestinal microorganisms primarily by disrupting the cell membrane integrity,causing intracellular substance leakage and bacterial death.
关键词
麦芽醇提物/肠道微生物/抑菌机制Key words
alcoholic extract of malt/gut microbe/antibacteria mechanism引用本文复制引用
出版年
2024
NETL
NSTL
万方数据