基于UPLC-Q-Orbitrap-HRMS技术的葛根-丹参药对化学成分分析
Chemical components of Gegen-Danshen herb pair by UPLC-Q-Orbitrap-HRMS
卢洋 1邓小惠 1吴梦瑶 2陈萱 3陈祥雯 3杨宇辉 3曹纪亮 3万丽2
作者信息
- 1. 成都中医药大学药学院/现代中药产业学院,成都 611137;深圳技术大学药学院,广东 深圳 518118
- 2. 成都中医药大学药学院/现代中药产业学院,成都 611137
- 3. 深圳技术大学药学院,广东 深圳 518118
- 折叠
摘要
目的 采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap-HRMS)技术分别对以水和50％甲醇溶液为溶剂提取的葛根-丹参药对样品进行化学成分分析.方法 采用 Thermo Scientific Hypersil GOLD C18 色谱柱(150 mm×2.1 mm,1.9 μm),以 0.1％甲酸水-0.1％甲酸乙腈溶液为流动相进行梯度洗脱,流速为0.3 mL·min-1,柱温为30℃.采用加热电喷雾离子源(H-ESI),正负离子检测模式.结果 从水提和50％甲醇醇提样品溶液中均鉴定出104个化合物,其中103个化合物为共有成分,各有1个差异性成分,分别为水提样品中的刺槐苷与50％甲醇醇提样品中的降丹参酮(或其同分异构体).共鉴定出的105个化合物,包括黄酮类成分36个、苯丙素类成分20个、萜类成分24个和其他类成分25个.结论 本研究首次采用UPLC-Q-Orbitrap-HRMS技术对葛根-丹参药对的主要成分进行鉴定,可为葛根-丹参药对的药效成分研究提供科学依据.
Abstract
Objective To analyze the chemical components of Gegen-Danshen herb pair extracted with water and 50％methanol solution by UPLC-Q-Orbitrap-HRMS.Methods Thermo Scientific Hypersil GOLD C18 column(150 mm × 2.1 mm,1.9 μm)was used for gradient elution with 0.1％formic acid water-0.1％formic acid acetonitrile solution as the mobile phase.The flow rate was 0.3 mL·min-1 and the column temperature was 30 ℃.A heated electrospray ionization(H-ESI)source was used for HRMS analysis in both positive and negative ion modes.Results In both water and 50％methanol extract samples,104 compounds were tentatively identified:103 common components,and one differentials component,namely acacetin in the water extract and nortanshinone(or its isomers)in 50％methanol extract.Among the identified 105 compounds,there were 36 flavonoids,20 phenylpropanoids,24 terpenoids and 25 other components.Conclusion The main components of Gegen-Danshen herb pair are characterized by UPLC-Q-Orbitrap-HRMS for the first time,which provides reference for the discovery of bioactive components in Gegen-Danshen herb pair.
关键词
葛根-丹参药对/UPLC-Q-Orbitrap-HRMS/化学成分Key words
Gegen-Danshen herb pair/UPLC-Q-Orbitrap-HRMS/chemical component引用本文复制引用
出版年
2024
NETL
NSTL
万方数据