Chemical components of Gegen-Danshen herb pair by UPLC-Q-Orbitrap-HRMS
Objective To analyze the chemical components of Gegen-Danshen herb pair extracted with water and 50％methanol solution by UPLC-Q-Orbitrap-HRMS.Methods Thermo Scientific Hypersil GOLD C18 column(150 mm × 2.1 mm,1.9 μm)was used for gradient elution with 0.1％formic acid water-0.1％formic acid acetonitrile solution as the mobile phase.The flow rate was 0.3 mL·min-1 and the column temperature was 30 ℃.A heated electrospray ionization(H-ESI)source was used for HRMS analysis in both positive and negative ion modes.Results In both water and 50％methanol extract samples,104 compounds were tentatively identified:103 common components,and one differentials component,namely acacetin in the water extract and nortanshinone(or its isomers)in 50％methanol extract.Among the identified 105 compounds,there were 36 flavonoids,20 phenylpropanoids,24 terpenoids and 25 other components.Conclusion The main components of Gegen-Danshen herb pair are characterized by UPLC-Q-Orbitrap-HRMS for the first time,which provides reference for the discovery of bioactive components in Gegen-Danshen herb pair.
Gegen-Danshen herb pairUPLC-Q-Orbitrap-HRMSchemical component
NETL
NSTL
万方数据
