Determination of related substances in folic acid tablets by HPLC
Objective To establish an HPLC method to determine the related substances in folic acid tablets.Methods The determination was performed on a Besil C18-B column(4.6 mm × 250 mm,3.5μm).The mobile phase consisted of phosphate buffered solution and methanol with gradient elution.The flow rate was 0.8 mL·min-1,the column temperature maintained at 35 ℃,and the detection wavelength was 280 nm.Results The resolutions between the peaks of folic acid and 6 known impurities were good.The content of folic acid in three batches samples showed that the known impurities and the maximum single unknown impurity and the total impurity complied with the quality standards.Conclusion The method is simple,accurate,and can be used for the determination of related substances in folic acid tablets.
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