Content of 6 flavonoids in medicinal parts of Dasiphora fruticosa(L.)Rydb
Objective To establish a method to determine the content of catechin,quercetin-3-O-(6"-galloyl)-β-D-galactopyranoside,hyperoside,isoquercitrin,avicularin and tiliroside in 4 medicinal parts of Dasiphora fruticosa(L.)Rydb,including leaves,flowers,fruits and branches,and to compare the content in different medicinal parts.Methods Agilent Poroshell 120 PFP column(4.6 mmX 150 mm,2.7 μm)was used.Acetonitrile-0.1％phosphoric acid aqueous solution was used as the mobile phase for gradient elution,with the switching detection wavelength of 280 nm and 354 nm.The column temperature was 30 ℃,and the flow rate was 0.8 mL·min-1.The histogram and stacked column chart were plotted to characterize the content of 6 chemical components in different medicinal parts.The Spearman correlation analysis of the content of chemical components was conducted with"Chiplot"mapping platform.Results The total content of the 6 chemical components in different parts was as follows:flowers＞leaves＞fruits＞branches.Spearman correlation analysis showed a strong positive correlation between the content of isoquercitrin and hyperoside,isoquercitrin and tiliroside,hyperoside and tiliroside,with correlation coefficients of 0.98,0.93 and 0.93,respectively.Conclusion The study provides a basis for the quality control,clinical application,and rational development and utilization of Dasiphora fruticosa.
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