Objective To determine the antitussive,expectorant and antiasthmatic effects of the decoction of Phragmites communis,to examine the active components and mechanism of action by UPLC-MS/MS combined with network pharmacology.Methods The antitussive,expectorant and antiasthmatic effects of the decoction were respectively detected by ammonia coughing method,phenolsulfonphthalein excretion test and OVA sensitization and excitation in mice.UPLC-MS/MS technologies were used to identify the components of the decoction.Network pharmacology was used to analyze the active ingredients,core targets and pathways of the antitussive,expectorant and antiasthmatic effects.Results Compared with the control group,the decoction at 5～10 g·kg-1 greatly prolonged the cough latency period,while that at 2.5～10 g·kg-1 reduced the times of coughing in mice.Inflammatory factor assay and tissue staining showed that the group at 5～10 g·kg-1 obviously increased tracheal phenol red excretion in mice.The decoction at 2.5～10 g·kg-1 decreased the levels of IgE,IL-4,IL-5,and IL-13 and elevated the levels of IFN-γ,improved the inflammatory infiltrates in the lungs,and decreased the area of positive staining for PAS and α-SMA in asthmatic mice.Totally 30 chemical components were identified by UPLC-MS/MS.Through network pharmacological analysis,11 potentially active chemical components and 182 targets were obtained,including luteolin,baicalein and naringenin.The number of intersecting targets generated between the three diseases and decoction was 103,61,and 94,respectively.Cough,sputum and asthma all involved GO biological processes such as apoptosis,response to xenobiotic stimuli,and related signaling pathways such as apoptosis and IL-17.Conclusion The decoction may inhibit the inflammatory responses by acting on the IL-17 pathway to exert its antitussive,expectorant and antiasthmatic effects.
维普
万方数据
