Objective To establish an HPLC fingerprints of Guangchenpi(dried tangerine peel)standard decoction and determine the quality correlation with its herbs and decoction pieces.Methods Totally 15 batches of Guangchenpi herbs,decoction pieces and standard decoction were analyzed by high performance liquid chromatography(HPLC).Their fingerprints were established and analyzed for similarity with Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2012 version).The four active ingredients(hesperidin,nobiletin,tangeretin and D-limonene)were quantified.Results HPLC fingerprints of Guangchenpi,its decoction pieces and its standard decoction were obtained,and 18,18 and 16 common peaks were identified.The similarity of decoction pieces was 0.994 when compared with the control fingerprint profiles of its herbs,while the similarity of standard decoction was 0.833.In addition,the mean value of the yield rate in its standard decoction was(43.2±0.1)％,and the mean values of the transfer rates of hesperidin,nobiletin,tangeretin and D-limonene were(19.3±4.9)％,(25.3±4.0)％,(16.6±2.5)％,and(2.2±2.2)％.Conclusion The fingerprints comprehensively reflect the similarity in chemical compositions of Guangchenpi herbs,decoction pieces and standard decoction.The components of standard decoction,including hesperidin,nobiletin and tangeretin,has good positive correlation with that of the herbs and decoction pieces,providing reference for the production,preparation and quality control of Guangchenpi standard decoction,formula granules and other preparations.
维普
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