摘要
目的 研究白背叶楤木Aralia chinensis var.nuda芽苞乙醇提取物乙酸乙酯部位芳香族化合物及其体外抗炎活性.方法 白背叶楤木芽苞70％乙醇提取物乙酸乙酯部位浸膏采用硅胶柱色谱分离法、RP-18反相色谱分离法、薄层色谱法、凝胶过滤层析法、半制备高效液相色谱法分离.根据理化性质及核磁共振波谱数据鉴定化合物结构.使用Griess法测定化合物对脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7产生一氧化氮(NO)的抑制活性.结果 从中分离得到14个芳香族化合物,分别鉴定为松脂素(1)、3'-O-去甲基表松脂素(2)、(+)-表松脂醇(3)、绿原酸甲酯(4)、绿原酸乙酯(5)、3,4-二咖啡酰基奎宁酸甲酯(6)、1-O-caffeoylglycerol(7)、drypetesinB(8)、papuline(9)、高山黄芩素(10)、红花素(11)、trichocarpine(12)、2-(3',4'-二羟苯基)-1,3-胡椒环-5-醛(13)、4-methoxy-1,2-dihydrocyclobutabenzene(14).体外抗炎活性研究结果表明,化合物13对LPS诱导的RAW264.7细胞NO释放有较强抑制活性,在50μmol·L-1浓度下,NO生成抑制率为(59.28±1.24)％.结论 化合物1～2、4～14为首次从楤木属植物中分离得到,化合物1～14为首次从白背叶楤木中分离得到,化合物13具有良好的体外抗炎活性.
Abstract
Objective To determine the aromatic compounds and anti-inflammation activities in vitro of ethanol extract of the bud of Aralia chinensis var.nuda.Methods The 70％ethanol extract from the bud of Aralia chinensis var.nuda was separated by silica gel column chromatography,RP-18 reversed-phase chromatography,thin-layer chromatography,gel filtration chromatography and semi-preparative high performance liquid chromatography.According to the physical and chemical properties and NMR data,the structures of the compounds were identified.The inhibition activities of the compounds on NO production in LPS-induced mouse macrophages RAW264.7 cells were evaluated by Griess assay.Results Totally 14 aromatic compounds were isolated and identified as rosin(1),3'-O-demethylepipinoresin(2),(+)-epiterpineol(3),methyl chlorogenic acid(4),ethyl chlorogenic acid(5),3,4-diccaffeoyl quinic acid methyl ester(6),1-O-caffeoylglycorol(7),drypetesin B(8),papuline(9),baicalein(10),carthamin(11),trichocarpine(12),2-(3',4'-dihydroxyphenyl)-1,3-piperonal-5-aldehyde(13),and 4-methoxy-1,2-dihydrocyclobutanzene(14).Compounds 13 exhibited strong inhibition on LPS-induced RAW264.7 cells,with the inhibition rates of NO production at(59.28±1.24)％at the concentration of 50 μmol·L-1.Conclusion Compounds 1～2,4～14 have been isolated from this genus Aralia for the first time,and compounds 1～14 from Aralia chinensis var.nuda for the first time.Compound 13 exhibits great anti-inflammation activities in vitro.
基金项目
国家自然科学基金(82260832)
国家自然科学基金(81860760)
云南省中医联合专项(202101AZ0 70001-159)
云南省重大科技专项(202002AA100007)
维普
万方数据