Aromatic compounds from ethyl acetate fraction of Aralia chinensis var.nuda and their anti-inflammation activities in vitro
Objective To determine the aromatic compounds and anti-inflammation activities in vitro of ethanol extract of the bud of Aralia chinensis var.nuda.Methods The 70％ethanol extract from the bud of Aralia chinensis var.nuda was separated by silica gel column chromatography,RP-18 reversed-phase chromatography,thin-layer chromatography,gel filtration chromatography and semi-preparative high performance liquid chromatography.According to the physical and chemical properties and NMR data,the structures of the compounds were identified.The inhibition activities of the compounds on NO production in LPS-induced mouse macrophages RAW264.7 cells were evaluated by Griess assay.Results Totally 14 aromatic compounds were isolated and identified as rosin(1),3'-O-demethylepipinoresin(2),(+)-epiterpineol(3),methyl chlorogenic acid(4),ethyl chlorogenic acid(5),3,4-diccaffeoyl quinic acid methyl ester(6),1-O-caffeoylglycorol(7),drypetesin B(8),papuline(9),baicalein(10),carthamin(11),trichocarpine(12),2-(3',4'-dihydroxyphenyl)-1,3-piperonal-5-aldehyde(13),and 4-methoxy-1,2-dihydrocyclobutanzene(14).Compounds 13 exhibited strong inhibition on LPS-induced RAW264.7 cells,with the inhibition rates of NO production at(59.28±1.24)％at the concentration of 50 μmol·L-1.Conclusion Compounds 1～2,4～14 have been isolated from this genus Aralia for the first time,and compounds 1～14 from Aralia chinensis var.nuda for the first time.Compound 13 exhibits great anti-inflammation activities in vitro.
Aralia chinensis var.nudaaromatic compoundisolation and identificationanti-inflammation activity
万方数据
维普
