Anti-tumor effect of saponins from Silene viscidula Franch.based on network pharmacology and in vitro experiments
Objective To determine the anti-tumor mechanism of saponins in Silene viscidula Franch.based on network pharmacology,molecular docking and in vitro experiments.Methods Network pharmacology was used to predict the key components and potential targets of saponins from Silene viscidula Franch.for anti-tumor effects.Molecular docking was used for validation.In vitro cell experiments verified the inhibitory effect of active saponins in Silene viscidula Franch.on various tumor cells.Results Network pharmacology screening identified 8 active components and 16 drug-disease co-action targets of saponins from Silene viscidula Franch..GO enrichment analysis identified 11 cell components,35 biological processes,and 16 molecular functions related to tumors.KEGG enrichment analysis identified 7 pathways related to tumors(P＜0.05),including nitrogen metabolism and cancer pathways.Molecular docking showed good binding activity of sinocrassuloside Ⅸ with key targets.In vitro experiments showed that saponins from Silene viscidula Franch.had a strong inhibitory effect on the tumor growth.Conclusion Saponins from Silene viscidula Franch.have significant in vitro anti-tumor effects,whose mechanism may be through pathways such as nitrogen metabolism and cancer pathways,as well as related targets.The study provides theoretical basis for the study of anti-tumor mechanism and clinical application of Silene viscidula Franch..
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