Screening of hub genes in children with ulcerative colitis and prediction of potential therapeutic drugs
Objective To identify differentially expressed genes(DEGs)in the colonic tissues of children with ulcerative colitis(UC)and to predict potential therapeutic drugs and related mechanism.Methods GSE126124 dataset from the GEO database was used to comprehensively screen for hub genes associated with UC in children.The acute UC mouse model was established by the dextran sulfate sodium(DSS),and the mRNA expression levels of hub genes were detected by qPCR.Subsequently,the Connectivity Map(CMAP)database was leveraged to identify candidate therapeutic drugs for UC.A protein-protein interaction network was established to elucidate the interactions between drug targets and disease-related genes,and the obvious gene module was extracted for further analysis.Go analysis and KEGG pathway enrichment analysis were used to determine the potential mechanism of predicted drug candidates.Results Totally 52 DEGs were identified in the colon tissues of children with UC.Notably,the mRNA levels of the hub genes,including DEFA6,DEFA5,CXCL1,MMP1,MMP7,DEFB4A,SAA1 and LCN2,were markedly elevated in the DSS-induced UC model.Prunetin,a natural active component screened by CMAP,exerted a therapeutic effect on UC by regulating IL-17,NF-κB and other signal pathways.Conclusion The identified DEGs may serve as potential therapeutic targets for UC.Furthermore,prunetin may become as a promising therapeutic drug for the treatment of UC.
万方数据
维普
