Content determination of 7 components in Shenshuai mixture by HPLC
Objective To establish an HPLC method to determine the content of 7 components in Shenshuai mixture.Methods The chromatographic column was Kromasil 100-5-C18(4.6 mm×250 mm,5 μm);the mobile phase was acetonitrile(A)-0.2％formic acid(B)with gradient elution:0～5 min,12％～14％A;5～25 min,14％～18％A;25～50 min,18％～21％A;50～75 min,21％～30％A;75～85 min,30％～40％A;85～95 min,40％～60％A;and 95～100 min,60％～12％A.The flow rate was 1.0 mL·min-1.The detection wavelength was 260 nm and the column temperature was 25 ℃.Results The mass concentrations of calycosin 7-O-β-D-glucopyranoside,neoastilbin,astilbin,hesperidin,calycosin,formononetin and nobiletin had good linearity at 2.01 to 10.03 μg·mL-1(r=0.999),8.64 to 43.2 μg·mL-1(r=0.995),5.25 to 26.25μg·mL-1(r=0.999),26.67 to 133.34 μg·mL-1(r=0.999),0.89 to 4.43 μg·mL-1(r=0.999),0.48 to 2.42 μg·mL-1(r=0.999)and 0.48 to 2.42 μg·mL-1(r=0.999).The RSDs of precision,repeatability and stability(24 h)were all lower than 3％.The average recoveries were 98.81％,105.58％,100.32％,104.68％,106.65％,100.28％,and 100.44％,and the RSDs were all lower than 3％,respectively.Conclusion This method is simple,repeatitive and exclusive,which can be used to determine multiple components in Shenshuai mixture.
万方数据
维普
