基于UPLC-LTQ-Orbitrap-MS的二十五味大汤丸化学成分分析
Chemical composition analysis of Twenty-five Flavor Soup pills based on UPLC-LTQ-Orbitrap-MS
严志宏 1许金娣 1索朗次仁 2张臣 1张宁方 1罗珍 2杨雅雯1
作者信息
- 1. 江西中医药大学,南昌 330004
- 2. 西藏藏医药大学,拉萨 850000
- 折叠
摘要
目的 采用UPLC-LTQ-Orbitrap-MS法对二十五味大汤丸的化学成分进行分析鉴定.方法 利用超高效液相色谱-线性离子阱-静电场轨道阱高分辨质谱技术(UPLC-LTQ-Orbitrap-MS)进行检测,正、负离子模式采集数据,采用TraceFinder4.1软件筛查目标化合物,分析化合物特征碎片离子,结合相关文献对数据进行系统分析和各成分的中药来源进行归属.结果 在正、负离子模式下共鉴定出102个化合物,包括黄酮类30个,有机酸类22个,萜类及三萜类11个,苯丙素类17个,鞣质类10个,其他类12个.结论 应用UPLC-LTQ-Orbitrap-MS技术可快速有效、全面地表征二十五味大汤丸中的主要化学成分,为深入揭示其药效物质及作用机制提供依据.
Abstract
Objective To identify the chemical components of Twenty-five Flavor Soup pills by UPLC-LTQ-Orbitrap-MS method.Methods The detection was carried out using ultra performance liquid chromatography-linear ion trap-electrostatic field orbitrap high-resolution mass spectrometry(UPLC-LTQ-Orbitrap-MS),and the data were collected in the positive and negative ion modes,and the target compounds were screened using TraceFinder 4.1 software to analyse the compound characteristic fragment ions,combined with the relevant literature for systematic analysis of the data and the attribution of the traditional Chinese medicine sources of each component.Results Totally 102 compounds were identified in the positive and negative ion modes,including 30 flavonoids,22 organic acids,11 terpenes and triterpenes,17 phenylpropanoids,10 tannins,and 12 other compounds.Conclusion The application of UPLC-LTQ-Orbitrap-MS technology can rapidly,effectively and comprehensively characterise the main chemical components in Twenty-five Flavor Soup pills,and provide the basis for the in-depth revelation of its pharmacological substances and mechanism of action.
关键词
二十五味大汤丸/UPLC-LTQ-Orbitrap-MS/化学成分/裂解规律Key words
Twenty-five Flavor Soup pill/UPLC-LTQ-Orbitrap-MS/chemical component/cracking regularity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据