Objective To determine the chemical components of Atractylodes japonica Koidz.ex Kitam.Methods Dried rhizome of Atractylodes japonica Koidz.ex Kitam was used.The dichloromethane part separated and purified from the n-butanol part of its 75％ethanol extract.The structure of the resulting compounds was identified by normal-phase silica gel chromatography,reversed-phase ODS,HPLC,and other spectroscopic techniques.The antitumor activity was determined for 14 compounds using human gastric cancer cells MGC-803 and human cervical cancer cells HeLa.Results Totally 14 compounds were obtained from Atractylodes japonica Koidz.ex Kitam and identified as deoxyschizandrin(1),(7S,8R)-dihydrodehydrodiconiferyl alcohol-9'-O-β-D-glucopyranoside(2),(7S,8R)-dehydrodiconiferyl alcohol 9'-β-glucopyranoside(3),artemordolignan glycoside A(4),secoisolariciresinol-9-O-β-D-glucopyranoside(5),2-methoxy-4-(2-propenyl)phenyl-β-D-glucopyranoside(6),1,2-di-O-β-D-glucopranosyl-4-allylbenzene(7),atractylenolideⅠ(8),atractylenolide Ⅱ(9),isoatractylenolide Ⅰ(10),atractylenolid Ⅲ(11),atractylenother(12),biatractylenolide(13),and biepiatractylenolide(14).Compounds 1,8～14 were isolated from the dichloromethane fraction while compounds 2～7 were isolated from the n-butanol fraction.The MTT experiments showed that compounds 1,8～14 had certain anti-tumor activity against HeLa cells,and compounds 8～11,13 had certain anti-tumor activity against MGC-803.Conclusion Totally 14 compounds have been obtained.Compounds 1～7 have been isolated from this medicinal part for the first time,several compounds showed certain anti-tumour activity.
Atractylodes japonica Koidz.ex Kitamchemical constituentextraction and separationstructural identificationanti-tumor activity
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