关苍术不同极性部位化学成分研究及其抗肿瘤活性研究
Chemical compositions of different polar parts of Atractylodes japonica and its antitumor activity
孙杰 1王静 1孙朝 1王萍 2方振兴 3于海龙 4王道深 1周媛媛1
作者信息
- 1. 黑龙江中医药大学教育部北药基础与应用研究重点实验室,哈尔滨 150040
- 2. 黑龙江省中医药科学院,哈尔滨 150036
- 3. 黑龙江省科学院自然与生态研究所,哈尔滨 150040
- 4. 哈尔滨市产品质量综合检验检测中心,哈尔滨 150030
- 折叠
摘要
目的 研究菊科苍术属植物关苍术(Atractylodes japonica Koidz.ex Kitam)的化学成分.方法 使用关苍术干燥根茎,将其75％乙醇提取物中的二氯甲烷部位与正丁醇部位进行分离纯化,使用正相硅胶色谱、反相ODS和HPLC等方法,并使用各种波谱技术对所得化合物进行结构鉴定.使用人胃癌细胞MGC-803和人宫颈癌细胞HeLa对14个化合物进行抗肿瘤活性测定.结果 从中共得到14种化合物,通过NMR和HR-ESI-MS方法鉴定分别为:五味子甲素(1)、(7S,8R)-dihydrodehydrodiconiferyl alcohol-9'-O-β-D-glucopyranoside(2)、(7S,8R)-dehydrodiconiferyl alcohol 9'-β-glucopyranoside(3)、artemordolignan glycoside A(4)、开环异落叶松脂素-9-O-β-D-葡萄糖苷(5)、2-甲氧基-4-(2-丙烯基)苯基-β-D-吡喃葡萄糖苷(6)、1,2-di-O-β-D-glucopranosyl-4-allylbenzene(7)、白术内酯 Ⅰ(8)、白术内酯 Ⅱ(9)、表白术内酯 Ⅰ(10)、白术内酯Ⅲ(11)、atractylenother(12)、双白术内酯(13)、双表白术内酯(14).从二氯甲烷组分中分离得到化合物1,8～14,正丁醇组分中分离得到化合物2～7.MTT实验结果显示,化合物1,8～14对HeLa细胞具有一定的抗肿瘤活性,化合物8～11,13对MGC-803具有一定的抗肿瘤活性.结论 本次实验共得到14个化合物,其中化合物1～7均为首次从该药用部位中分离获得,部分化合物具有一定的抗肿瘤活性.
Abstract
Objective To determine the chemical components of Atractylodes japonica Koidz.ex Kitam.Methods Dried rhizome of Atractylodes japonica Koidz.ex Kitam was used.The dichloromethane part separated and purified from the n-butanol part of its 75％ethanol extract.The structure of the resulting compounds was identified by normal-phase silica gel chromatography,reversed-phase ODS,HPLC,and other spectroscopic techniques.The antitumor activity was determined for 14 compounds using human gastric cancer cells MGC-803 and human cervical cancer cells HeLa.Results Totally 14 compounds were obtained from Atractylodes japonica Koidz.ex Kitam and identified as deoxyschizandrin(1),(7S,8R)-dihydrodehydrodiconiferyl alcohol-9'-O-β-D-glucopyranoside(2),(7S,8R)-dehydrodiconiferyl alcohol 9'-β-glucopyranoside(3),artemordolignan glycoside A(4),secoisolariciresinol-9-O-β-D-glucopyranoside(5),2-methoxy-4-(2-propenyl)phenyl-β-D-glucopyranoside(6),1,2-di-O-β-D-glucopranosyl-4-allylbenzene(7),atractylenolideⅠ(8),atractylenolide Ⅱ(9),isoatractylenolide Ⅰ(10),atractylenolid Ⅲ(11),atractylenother(12),biatractylenolide(13),and biepiatractylenolide(14).Compounds 1,8～14 were isolated from the dichloromethane fraction while compounds 2～7 were isolated from the n-butanol fraction.The MTT experiments showed that compounds 1,8～14 had certain anti-tumor activity against HeLa cells,and compounds 8～11,13 had certain anti-tumor activity against MGC-803.Conclusion Totally 14 compounds have been obtained.Compounds 1～7 have been isolated from this medicinal part for the first time,several compounds showed certain anti-tumour activity.
关键词
关苍术/化学成分/提取分离/结构鉴定/抗肿瘤活性Key words
Atractylodes japonica Koidz.ex Kitam/chemical constituent/extraction and separation/structural identification/anti-tumor activity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据