Chemical compositions of different polar parts of Atractylodes japonica and its antitumor activity
Objective To determine the chemical components of Atractylodes japonica Koidz.ex Kitam.Methods Dried rhizome of Atractylodes japonica Koidz.ex Kitam was used.The dichloromethane part separated and purified from the n-butanol part of its 75％ethanol extract.The structure of the resulting compounds was identified by normal-phase silica gel chromatography,reversed-phase ODS,HPLC,and other spectroscopic techniques.The antitumor activity was determined for 14 compounds using human gastric cancer cells MGC-803 and human cervical cancer cells HeLa.Results Totally 14 compounds were obtained from Atractylodes japonica Koidz.ex Kitam and identified as deoxyschizandrin(1),(7S,8R)-dihydrodehydrodiconiferyl alcohol-9'-O-β-D-glucopyranoside(2),(7S,8R)-dehydrodiconiferyl alcohol 9'-β-glucopyranoside(3),artemordolignan glycoside A(4),secoisolariciresinol-9-O-β-D-glucopyranoside(5),2-methoxy-4-(2-propenyl)phenyl-β-D-glucopyranoside(6),1,2-di-O-β-D-glucopranosyl-4-allylbenzene(7),atractylenolideⅠ(8),atractylenolide Ⅱ(9),isoatractylenolide Ⅰ(10),atractylenolid Ⅲ(11),atractylenother(12),biatractylenolide(13),and biepiatractylenolide(14).Compounds 1,8～14 were isolated from the dichloromethane fraction while compounds 2～7 were isolated from the n-butanol fraction.The MTT experiments showed that compounds 1,8～14 had certain anti-tumor activity against HeLa cells,and compounds 8～11,13 had certain anti-tumor activity against MGC-803.Conclusion Totally 14 compounds have been obtained.Compounds 1～7 have been isolated from this medicinal part for the first time,several compounds showed certain anti-tumour activity.
Atractylodes japonica Koidz.ex Kitamchemical constituentextraction and separationstructural identificationanti-tumor activity
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