Identification of isoflavone chemical constituents in Puerariae lobatae radix and related network pharmacology
Objective To determine isoflavone chemical constituents in Puerariae lobatae radix and their mechanisms of action in treating diabetes mellitus with network pharmacology.Methods Isoflavone compounds were isolated and purified from an 80％ethanol extract of Pueraria lobata with macroporous resin,C18 column chromatography,and preparative high-performance liquid chromatography.The structures were identified primarily through NMR and other spectral analyses.The potential targets and pathways for treating diabetes were predicted with databases such as PubChem and GeneCards.The core genes were subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.A"constituent-target-pathway-disease"network was establish wtih Cytoscape software.Results Totally 15 isoflavone compounds were isolated and identified:3'-methoxydaidzein(1),puerarin-4'-O-β-D-glucopyranoside(2),6"-O-malonyldaidzin(3),puerarin 6"-O-xyloside(4),glycitin(5),3'-methoxydaidzin(6),puerarin(7),3'-methoxypuerarin(8),daidzein(9),mirificin(10),mirificin-4'-O-glucoside(11),3'-hydroxymirificin(12),daidzein-7,4'-diglucoside(13),ononin(14),and dihydrodaidzin(15).Out of the 15 compounds,111 potential targets in Pueraria lobata for diabetes were screened,with compound 3 and compound 15 acting on the key targets CASP3 and PRKCA,respectively.Conclusion Compound 15 is a newly isolated isoflavone from the genus Pueraria.The network pharmacology research provides insights into the use of Pueraria lobata for diabetes mellitus.
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