Effect of Biejia Ruanmai decoction on learning and memory impairment in mice with chronic cerebral hypoperfusion
Objective To determine the therapeutic effect of Biejia Ruanmai decoction on learning and memory impairment in mice with chronic cerebral hypoperfusion(CCH)and its potential mechanism of action.Methods CCH mice models were established with the bilateral common carotid artery stenosis method.We evaluated the learning and memory abilities of the mice through the Morris water maze and novel object recognition tests.We detected the myelin damage and neuronal apoptosis in the mouse brain tissues through LFB,Nissl,NeuN,and TUNEL staining.The expression of NF-κB p65 in the mouse brain tissues was detected by immunofluorescence staining.The expression levels of NF-κB p65 and IKK-β in the mouse brain tissues were detected by Western blot.Results The Morris water maze test obviously improved the learning and memory in the high-dose Biejia Ruanmai group as compared with the model group(P＜0.05).After 6 weeks of treatment,both the low-and high-dose Biejia Ruanmai groups significantly increased the novel object recognition index(P＜0.05,P＜0.01),with sustained improvement in the high-dose group after 12 weeks(P＜0.05).Histopathological examination indicated reduced myelin damage and neuronal apoptosis in the brain tissues of Biejia Ruanmai-treated mice.Immunofluorescence staining showed reduced NF-κB p65 expression in the microglia of the low-dose group(P＜0.05),and reduced CD1 lb and NF-κB p65 expression in the high-dose group(P＜0.05,P＜0.01).Western blot confirmed that the high-dose BJRM group significantly downregulated NF-κB p65 and IKK-β expression in the brain tissues(P＜0.05,P＜0.01).Conclusion Biejia Ruanmai decoction may mitigate brain myelin and neuronal damage in CCH mice through the NF-κB pathway,thereby ameliorating cognitive dysfunction.
Biejia Ruanmai decoctionchronic cerebral hypoperfusionlearning and memory impairmentapoptosismyelin sheath injuryNF-κB
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