Objective To establish a quantitative analysis of multi-components by single marker(QAMS)to determine 6 components in Chaihu Muli granules.Methods Waters C18 reverse chromatographic column was used.The mobile phase was acetonitrile-0.5％phosphoric acid solution with gradient elution;the flow rate was 0.8 mL·min-1;the column temperature was 25 ℃;and the detection wavelength was 274 nm.Baicalin was used as the internal standard,a QAMS method was established to simultaneously determine the content of baicalin,scutellarin,oroxylin A-7-O-β-D-glucuronide,wogonoside,baicalein and wogonin.Results The 6 components showed good linearity in their respective concentration ranges(r≥0.999).With baicalin as the internal standard,the relative correction factors of scutellarin,oroxylin A-7-O-β-D-glucuronide,wogonoside,baicalein and wogonin were 1.80,1.14,0.91,0.69 and 0.62,respectively.The 6 components in 7 batches of Chaihu Muli granules were 7.80～8.61,0.32～0.36,0.94～1.09,1.77～1.83,0.17～0.18,and 0.12～0.13 mg·g-1.Conclusion The QAMS method is simple,economical and feasible,which provides a basis for the quality control of Chaihu Muli granules.
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