HPLC determination of the content of protopine in Hypecoum leptocarpum and its influence factors at different altitudes
Objective To establish an HPLC method to determine the content of protopine in 31 batches of Xizang medicine Hypecoum leptocarpum from different origins and altitudes.Methods A ZORBAX SB-C18 column(4.6 mm × 250 mm,5 μm)was used with a mobile phase of methanol-0.1 mol·L-1 ammonium acetate solution(32∶68,V/V).The injection volume was 20 μL,the flow rate was 0.8 mL·min-1,the column temperature was 35 ℃,and the detection wavelength was 289 nm.Results There was a significant difference in the protopine content in Hypecoum leptocarpum from different origins,with higher protopine content in middle to high altitudes,but low levels in high altitudes.Conclusion The method can be used for the determination of protopine content.The difference in protopine content at different altitudes provides reference for the cultivation,development,and utilization of the medicinal herb.
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