阿那日五味散HPLC指纹图谱和多指标含量测定的研究
HPLC fingerprint and multi-index content determination of Anari Wuwei power
张玉婷 1何春龙 1莎日娜 2王焕芸 1刘爽 2霍婷2
作者信息
- 1. 内蒙古医科大学药学院,呼和浩特 010110
- 2. 内蒙古医科大学第二附属医院,呼和浩特 010110
- 折叠
摘要
目的 建立蒙药阿那日五味散指纹图谱和多指标含量测定方法.方法 采用Sepax HP-C18(4.6 mm×250 mm,5 μm)色谱柱,甲醇-0.1％磷酸溶液为流动相,梯度洗脱;流速为1 mL·min-1;检测波长为275 nm;柱温为30 ℃;进样量为10 μL;建立15批阿那日五味散的指纹图谱,并采用一测多评法测定其中5种成分的含量.结果 阿那日五味散HPLC指纹图谱确定12个共有峰,指认出原儿茶酸、没食子酸、安石榴苷、肉桂醛、鞣花酸、胡椒碱、槲皮素7个成分,通过一测多评法计算得到5个成分的含量值与外标法比较,15批阿那日五味散含量测定结果一致.经方法学验证,各成分在各自线性范围内与峰面积线性关系良好(r≥0.9990),加样回收率为95.0％～105.0％.结论 所建立的指纹图谱和多指标含量测定方法准确、高效,特征性强,可为蒙药阿那日五味散的质量控制提供参考.
Abstract
Objective To establish the fingerprint and multi-index content determination method for Mongolian medicine Anari Wuwei power,and provide a basis for its quality control.Methods Sepax HP-C18(4.6 mm × 250 mm,5 μm)chromatographic column was used with methanol 0.1％phosphoric acid solution as the mobile phase in gradient elution.The flow rate was 1 mL·min-1,the detection wavelength was 275 nm,the column temperature was 30 ℃,and the injection volume was 10 μL.The fingerprint spectra of 15 batches of Anari Wuwei power were established.Quantitative analysis of multi-components by single-marker was used to measure the content of 5 components.Results The HPLC fingerprint of Anari Wuwei powder identified 12 common peaks,and 7 components,namely protocatechuic acid,gallic acid,pomegranate glycoside,cinnamaldehyde,ellagic acid,piperine,and quercetin.The content of 5 components was measured with quantitative analysis of multi-components by single-marker and was compared with that of the external standard method.The 15 batches of Anari Wuwei powder were consistent.Through methodological verification,the linear relationship was good(r≥0.9990),and the recovery ranged from 95.0％to 105.0％.Conclusion The fingerprint and multi-index content determination method are accurate,efficient,and highly characteristics,which can provide reference for the quality control of Anari Wuwei power.
关键词
阿那日五味散/指纹图谱/一测多评法/高效液相色谱法Key words
Anari Wuwei power/fingerprint/quantitative analysis of multi-components by single-marker/high performance liquid chromatography引用本文复制引用
出版年
2024
NETL
NSTL
万方数据