首页|RVM致炎细胞因子上调导致5-HT释放参与调控慢性术后疼痛

RVM致炎细胞因子上调导致5-HT释放参与调控慢性术后疼痛

扫码查看
原文链接
  • 万方数据
  • 维普
[目的]探讨延髓头端腹内侧部(RVM)内致炎细胞因子肿瘤坏死因子α(TNFα)和白介素-1β(IL-1β)释放增多导致5-羟色胺(5-HT)向脊髓释放增多在慢性术后疼痛(CPSP)模型中的作用.[方法]按照随机方式将SD 大鼠进行如下分组:对照组、皮肤/肌肉切开和牵拉(SMIR)(1d,7d)组、SMIR+RVM内注射TNFα或IL-1β中和性抗体组、SMIR+RVM内注射TNFα或IL-1β组、SMIR+RVM内注射溶剂组,用up-down 方法测量大鼠50%机械刺激撤足阈值,免疫组化检测RVM内TNFα或IL-1β表达情况,酶联免疫吸附测定法(ELISA)方法观察RVM及脊髓背角内5-HT含量的变化.[结果]SMIR可引起大鼠机械痛敏,表现为50%机械刺激撤足阈值下降,持续至少3周.SMIR后,RVM内神经元和星形胶质细胞中的TNFα和IL-1 ß表达上调.TNFα 或IL-1β 中和抗体(每天一次行SMIR术前30 min给予,共4次))微量注射入RVM可阻断SMIR引起的50%机械刺激撤足阈值下降,并可降低RVM及脊髓背角5-HT的含量.正常动物RVM内注射TNFα和IL-1 ß也可导致50%机械刺激撤足阈值下降,且引起RVM及脊髓内5-HT含量增加.[结论]RVM中致炎细胞因子增多可能通过引起5-HT释放增多参与调控SMIR后慢性术后疼痛的发生发展.
Up-regulation of Proinflammatory Cytokines in Rostral Ventromedial Medulla Contributes to Chronic Postsurgical Pain by Promoting 5-HT Release
[Objective]To investigate the role of proinflammatory cytokines tumor necrosis factor alpha(TNFα)and interleukin-1β(IL-1β)in rostral ventromedial medulla(RVM)in chronic postsurgical pain(CPSP)induced by skin/muscle incision and retraction(SMIR).[Methods]SD rats were randomly divided into 5 groups:① Sham group;② SMIR group;③ SMIR+TNFα/IL-1β neutralizing antibody group;④ SMIR+TNFα/IL-1β group and ⑤ SMIR+vehicle group.50%paw mechanical withdrawal threshold(MWT)was measured by the up-down method,immunofluroscence was used to detect the TNFα and IL-1β expression and ELISA for the 5-Hydroxytryptamine(5-HT)level.[Results]SMIR elicited persistent nociceptive sensitization,upregulated TNFα and IL-1β expression in RVM neurons and astrocytes.Microinjec-tion of TNFα or IL-1β neutralizing antibody into RVM inhibited the development of nociceptive sensitization and de-creased the level of 5-HT in both RVM and spinal dorsal horn.While microinjection of recombinant TNFα or IL-1β into RVM enhanced the development of nociceptive sensitization and increased the level of 5-HT in both RVM and spinal dor-sal horn.[Conclusion]Up-regulation of proinflammatory cytokines in RVM may contribute to SMIR induced CPSP by pro-moting 5-HT release.

chronic postoperative pain(CPSP)rostral ventromedial medulla(RVM)tumor necrosis factor alpha(TNFα)interleukin-1β(IL-1β)5-Hydroxytryptamine(5-HT)

代娟丽、王臻、董超雄、李玉莹、魏绪红

展开 >

华中科技大学协和深圳医院,广东 深圳 518052

深圳职业技术大学,广东 深圳 518055

中山大学中山医学院生理教研室,疼痛研究中心,广东 广州 510080

慢性术后痛 延髓头端腹内侧部 肿瘤坏死因子α 白介素1β 5-羟色胺

国家自然科学基金国家自然科学基金广东省自然科学基金深圳市南山区科技计划项目深圳职业技术大学校级项目

81870969814712502019A1515011855NS20210786023310001K

2024

10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240004.005

中山大学学报(医学科学版)
中山大学

中山大学学报(医学科学版)

CSTPCD北大核心
影响因子：1.608
ISSN：1672-3554
年,卷(期)：2024.45(1)
  • 1