Expression of GDNF and AR in Testicular Peritubular Cells of Surgery-induced Cryptorchidism Mice
[Objective]To investigate the expression of glial cell line-derived neurotrophic factor(GDNF)and andro-gen receptor(AR)in testicular peritubular cells(TPCs)of cryptorchidism mouse models and explore the theoretical signif-icance of cryptorchidism-induced spermatogenesis dysfunction.[Methods]A total of 30 five-week-old male ICR rats were divided randomly by using random number table method into 6 groups.Cryptorchidism was surgically induced in 3 random-ly selected groups and the other 3 groups underwent sham surgery as the control groups.On days 4,7 and 14 after surgery,we harvested the mice testes of the 3 groups and their corresponding control groups,then measured the testicular volumes,analyzed the testicular histopathology and detected the mRNA and protein expression levels of AR and GDNF in TPCs by immunofluorescence,real-time PCR and Western blot.[Results]In normal control groups,on days 4,7 and 14 after sur-gery,the testicular volumes were(125.58±19.22)mm3,(123.45±20.12)mm3,(140.09±13.62)mm3,respectively.Clear layers of spermatogenic cells were well arranged and abundant sperm cells were found.Peritubular cells were morphologi-cally homogeneous,with slim-spindle appearance and normal cell thickness.The mRNA expression levels of AR were 1.00±0.05,1.06±0.07 and 1.19±0.13;GDNF mRNA 1.00±0.04,1.09±0.05,and 1.10±0.07.The protein expression lev-els of AR were 1.01±0.01,0.79±0.02 and 1.01±0.04;GDNF protein(18.68±0.43)pg/mL,(14.39±0.36)pg/mL and(16.88±0.37)pg/mL.In cryptorchidism groups,on days 4,7 and 14 after surgery,the testicular volumes were(115.64±3.91)mm3,(69.51±14.97)mm3 and(44.86±5.56)mm3,respectively.Spermatogenic cells were disorganized,seminifer-ous tubules were disrupted,peritubular cells shrank,bent and fractured.The mRNA expression levels of AR were 0.76±0.06,0.53±0.04,and 0.29±0.02;GDNF mRNA 0.72±0.05,0.42±0.02 and 0.30±0.03.The protein expression levels of AR were 0.54±0.02,0.98±0.04 and 0.31±0.01;GDNF protein(8.50±0.34)pg/mL,(17.44±0.32)pg/mL and(6.83±0.34)pg/mL.Statistically significant differences(P＜0.05)were found in 7-day and 14-day testicular volumes between control and cryptorchidism groups but not in the 4-day testicular volume(P＞0.05).Testicular volumes,AR and GDNF mRNA and protein expression in control groups had no statistically significant difference(P＞0.05),while those in crypt-orchidism groups showed a trend of gradual decline in the amount and the differences between groups were statistically sig-nificant(P＜0.05).[Conclusions]In surgery-induced cryptorchidism mice,after the induction,the expression of AR and GDNF in TPCs showed a gradual decrease over time.AR and GDNF play a major role in mediating the TPCs damage in cryptorchidism.This study provides a theoretical basis for mechanism researches of cryptorchidism-induced spermatogene-sis dysfunction.
万方数据
维普
