Bactericidal Effect of Selenium Nanoparticles Combined with Povidone-iodine on Pathogenic Bacteria in Surgical Site Infection
[Objective]To investigate the bactericidal effect of loaded multifunctional povidoneiodine-nanometer sele-nium(PVP-I@Se)disinfectant on Staphylococcus aureus(SA)and methicillin-resistant Staphylococcus aureus(MRSA),and to provide an experimental basis for the reduction of surgical site infection(SSI).[Methods]The control group was the povidone iodine(PVP-I)group with different concentrations of iodine(50,75,100,200 and 400 μg/mL).The PVP-I@Se group(experimental group)was the PVP-I group further supplemented with 2 μg/mL Selenium nanoparticles(SeNPs).Then we compared the bactericidal effect of the two groups of disinfectant solutions on SA and MRSA by examin-ing the minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),the shortest sterilization time at a concentration of 50 μg/mL iodine and the inhibition zone diameters at concentrations of 200 μg/mL and 400 μg/mL iodine.[Results]MIC values of PVP-I against SA and MRSA were both 79.17 μg/mL,and those of PVP-I@Se were 54.17 and 70.83 μg/mL,respectively.MBC values of PVP-I against SA and MRSA were 129.17 and 150.00 μg/mL,re-spectively,and those of PVP-I@Se were 70.83 and 87.50 μg/mL,respectively.At a concentration of 50 μg/mL iodine,the shortest sterilization time of PVP-I for SA and MRSA was 130 s and 140 s,respectively,and that of PVP-I@Se was 65 s and 75 s,respectively.At a concentration of 200 μg/ml iodine,the inhibition zone diameters of PVP-I for SA and MRSA were 7.67 mm and 8.33 mm,and those of PVP-I@Se were both 9.50 mm.At a concentration of 400 μg/mL iodine,the in-hibition zone diameters of PVP-I for SA and MRSA were 9.00 mm and 9.33 mm,and those of PVP-I@Se were 11.67 mm and 12.00 mm,respectively.[Conclusions]PVP-I with different concentrations of 50,75,100,200 and 400 μg/mL io-dine supplemented with 2 μg/mL SeNPs have better and faster bactericidal effect on SA and MRSA.When combined with SeNPs,PVP-I can enhance the bactericidal activity against SA and MRSA,but with better sensitizing effect on SA than MRSA and higher demand of iodine concentration(400 μg/mL)for sensitizing effect on MRSA.This study provides a theo-retical basis for selecting optimal concentration and action time of the disinfectant,thus reducing SSI.
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