[目的]探究电针对少弱精症大鼠精子质量、性激素水平以及睾丸组织中TGF-β1/smads信号通路中相关蛋白的影响.[方法]取40只SD大鼠,雄性,采用随机数字表法,分成空白组、模型组、电针组、阳性药物组,应用腺嘌呤灌胃法建立少弱精症大鼠模型,分别对电针组(每天电针中极、关元、足三里、三阴交,30 min/次,连续28 d)、阳性药物组(以10 mL/kg 左卡尼汀口服液灌胃,连续28 d)进行干预.治疗后检测各组大鼠精子数量、精子活力率、促卵泡生成激素(FSH)、黄体生成素(LH)、血清性激素睾酮(T)指标变化;采用HE染色法对睾丸组织进行形态学观察;采用Western Blot 检测睾丸组织中 TGF-β1/smads 信号通路相关蛋白的表达.[结果]模型组与空白组比较,精子数量、精子活力率均显著降低(P＜0.05),电针组和阳性药物组与模型组比较均有显著升高(P＜0.05).性激素水平比较:模型组与空白组相比,血清 T 水平显著降低(P＜0.05)、血清 FSH、LH 水平显著升高(P＜0.05);电针组和阳性药物组与模型组比较,血清 T 水平显著升高(P＜0.05)、血清 FSH、LH 水平显著降低(P＜0.05).HE 染色结果显示:模型组大鼠睾丸组织出现典型 AR 病理特征,电针组和阳性药物组睾丸组织均出现不同程度改善.Western Blot 检测显示:睾丸组织中模型组 TGF-β1/smads 通路 TGF-β、p-smad2、p-smad3 蛋白水平表达均显著升高(P＜0.05),电针组和阳性药物组与模型组比较 TGF-β、p-smad2、p-smad3 蛋白水平均显著降低(P＜0.05).[结论]电针能够提高少弱精子症大鼠精子数量和精子活力率,调节性激素水平,改善大鼠的生精环境,其机制可能与通过调节TGF-β1/smads信号通路影响睾丸生精过程有关.
Effect of Electricity Targeting TGF-β 1/smads Signaling in Oligoasthenthymic Rats
[Objective]To explore the effects of electricity on sperm quality,sex hormone levels,and related proteins in TGF-β 1/smads signaling in testicular tissue.[Methods]Forty male SD rats were randomly divided into blank group,model group,electroacupuncture group,and positive drug group.Adenine gavage method was used to establish the model,in the electroacupuncture group,rats received electroacupuncture treatment at the acupoints Zhongji,Guanyuan,Zusanli,and Sanyinjiao once daily for 30 minutes;in the positive drug group,rats were administered L-carnitine oral solution via gavage at a dosage of 10 mL/kg,both intervention groups underwent continuous treatment for 28 days.After treatment,the changes of sperm number,sperm motility rate,follicle-stimulating hormone(FSH),luteizing hormone(LH)and serum sex hormone testosterone(T)were detected by HE staining,and the expression of TGF-β-1/smads signaling pathway in testicular tissue was examined by Western Blot.[Results]Compared to the blank group,the model group showed a significant decrease in sperm count and sperm motility rate(P<0.05),both the electroacupuncture group and the positive drug group exhibited significant increases compared to the model group(P<0.05).Compared to the blank group,the model group had a significant decrease in serum testosterone(T)levels(P<0.05),and significant increases in serum FSH and LH levels(P<0.05);in comparison to the model group,the electroacupuncture group and the positive drug group showed significant increases in serum T levels(P<0.05),and significant decreases in serum FSH and LH levels(P<0.05).The HE staining results showed typical pathological features of AR in the testicular tissue of model group rats,with varying degrees of improvement observed in electroacupuncture and positive drug group rats.Western Blot analysis revealed that in the model group,the protein levels of TGF-β,p-smad2,and p-smad3 in the TGF-β1/smads pathway in testicular tissue were significantly increased(P<0.05);in comparison to the model group,the electroacupuncture group and the positive drug group showed significant decreases in the protein levels of TGF-β,p-smad2,and p-smad3(P<0.05).[Conclusion]Acupuncture can improve the sperm number and sperm motility rate,regulate sex hormone level and improve the spermatogenic environment,and affect the spermatogenesis process via TGF-β 1/smads signaling pathway.
万方数据
维普
