摘要
建立甘草颗粒中甘草苷、甘草酸铵含量检测方法并进行指纹图谱研究.采用高效液相色谱法,色谱柱为 Eclipse Plus C18柱(4.6 mm×250.0 mm,5 µm),检测波长 237 nm,以乙腈(A)-0.05％磷酸(B)为流动相,梯度洗脱,流速为1.0 mL/min,柱温为30℃,进样量10 µL.结果显示,10批样品有12个共有峰,相似度大于0.901.经对照品比对,指认甘草苷和甘草酸铵两个指标性成分并对其进行定量分析,线性关系良好(R2＞0.999 8),平均加样回收率为99.67％～102.42％.该方法操作简单、快捷,结果准确,可以甘草颗粒中甘草苷、甘草酸铵含量的测定对甘草颗粒进行质量评价.
Abstract
The aim was to establish the determination method and fingerprint of liquiritin and ammonium glycyrrhizinate in Radix Glycyrrhizae granules.Methods High performance liquid chromatography(HPLC)was performed on Eclipse Plus C18 column(4.6 mm×250.0 mm,5 pm)with detection wavelength of 237 nm.Acetonitrile(A)-0.05％phosphoric acid(B)was used as mobile phase.Gradient elution was adopted,the flow rate was 1.0 mL/min,the column temperature chamber was 30℃,and the sample size was 10 μL.The results showed that there were 12 common peaks in 10 batches of samples,and the similarity was greater than 0.901.Two index components,liquiritin and ammonium glycyrrhizinate,were identified and quantitatively analyzed.The linear relationship was good(R2＞0.999 8),and the average recovery rate was 99.67％-102.42％.The experiment is simple,quick and accurate.It can be used to determine the content of liquiritin and ammonium glycyrrhizinate in Radix Glycyrrhizae granules and evaluate the quality of Radix Glycyrrhizae granules.
基金项目
贵州省科技厅项目(黔科合成果[2021]一般039)
贵州省科技厅项目(黔科合成果[2022]一般008)
贵州省农业农村厅项目(GZCYTX-0302)
维普
万方数据