Effects of Echinacea and its compound on antioxidant function,protease and HSP70 of heat stressed laying hen
To investigate the mechanism of Echinacea and its compound against heat stress,the effects of them on antioxidant activity,protease,and HSP70 were observed.250 60-week-old Hailan White laying hens were randomly divided into 5 groups.Echinacea compound group,Echinacea group,multiplex vitmin of electrolyte group and high temperature group were kept at 33℃-35℃.The first three groups were supplemented with 1.0％Echinacea compound,1.0％Echinacea and 0.1％multiplex vitmin of electrolyte in basal diets,respectively.And the normal temperature group was kept at 24℃-26℃.On 14 d,28 d,42 d and 56 d,the tissues of stomach,pancreas and spleen were collected to measure the activity of pepsin and trypsin,the index of antioxidant activity and HSP70.Echinacea compound,Echinacea and multiplex vitmin of electrolyte could restore the reduced pepsin activity in laying hens caused by heat stress,and the effect of Echinacea compound was significantly better than Echinacea and multiplex vitmin of electrolyte(P＜0.05).Echinacea compound and Echinacea could significantly increase trypsin activity(P＜0.05).Echinacea compound could significantly increase the activity of SOD and GSH-Px in the spleen(P＜0.05).Echinacea compound and multiplex vitmin of electrolyte could restore the level of T-AOC in spleen tissue.Meanwhile,Echinacea compound and Echinacea could improve the positive expression signal of HSP70 in the spleen.These results indicate that Echinacea compound and Echinacea can improve the antioxidant activity,protease levels,and HSP70 expression of heat stressed laying hens,and Echinacea compound has a better effect.
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维普
