In order to improve the quality standard of Qiqing Baidu granules and provide a basis for its quality evaluation,the characteristic chromatogram of Qiqing Baidu granules was established by RP-HPLC to identify the population characteristics of various components of Radix Scutellariae,Rhizoma Polygoni Cuspidati,Radix Isatidis and Folium Isatidis.The components of baicalin,polydatin and indatin were determined by referring to relevant literatures and pre-analysis tests.The chromatographic column was Waters XBridge C18(4.6 mm × 250.0 mm,3.5 μm),and acetonitrile was used as mobile phase A.Gradient elution was performed with 0.4％phosphoric acid solution as mobile phase B.PDA detector was used to scan the range of 190-450 nm in 3D,and the detection wavelength was determined to be 288 nm,the flow rate was 1.0 mL/min,and the column temperature was 30℃.The chromatogram showed 4 characteristic peaks,and the retention time was consistent with the corresponding reference peaks.The precision test,stability test and durability test proved that the method had high sensitivity,strong specificity and good repeatability.The chromatographic data of mixed reference solution and 6 batches of Qiqing Baidu granules samples were imported into the TCM Chromatographic Fingerprint Similarity Evaluation System(2012 edition).With S1 as the reference graph,the mean correlation coefficient method was used for multi-point correction,and the time window was 0.1 s for automatic matching.The similarity between 6 batches of Qiqing Baidu granules and the control graph was calculated.All of them were greater than 0.993.The corresponding 4 common peaks in the reference solution and Qiqing Baidu granules sample were compared by chromatogram and spectrogram,and all of them had good coincidence,which met the requirements of Chinese Veterinary Pharmacopoeia.The method saves reagents and time,is easy to operate and environmentally friendly,and can control the internal quality of Qiqing Baidu granules more effectively.
维普
万方数据
