Effects of water extract of Perilla meal on growth performance and intestinal health of yellow-feathered broilers
This experiment was conducted to investigate the effects of dietary Perila meal(PML)supplementation on growth performance,serum biochemical,antioxidantion and intestinal morphology of broilers.A total of 180 1-day-old yellow-feathered broilers were randomly assigned to 4 groups with 3 replicates per group and 15 birds per replicate.Broilers were fed a basal diet(control group),the basal diet supplemented with 0.25 g/kg(PMLL group),0.50 g/kg(PMLM group)or 1.00 g/kg(PMLH group)PML for 28 days.The broilers were provided the diets in ad libitum basis.Blood,immune organs and intestinal tissues were collected at 28 d,and the expression of genes,intestinal villus height and crypts depth were measured.The results showed as follows:1)compared with the CON group,the final weight,the mRNA expression levels of blocking protein-1(claudin-1)and blocking zonulin-2(ZO-2)in jejunal mucosa at 28 d(P<0.05)in PMLL group were all significantly increased(P<0.05);2)compared with the CON group,the final weight,the jejunal villus height,the ratio of jejunal villus height to crypt depth,the mRNA expression levels of mucin-2 and ZO-2 in jejunal mucosa,the mRNA expression levels of phosphorylated nuclear factor 2 related factor 2(Nrf-2),quinone oxidoreductase-1(NQO-1)and heme oxygenase-1(HO-1)in liver at 28 d in PMLM group were all increased significantly(P<0.05),but there was no significant difference in crypt depth between all groups(P>0.05);3)compared with the CON group,the levels of serum SOD and GSH-Px,the mRNA expression levels of Nrf-2,NQO-1 and HO-1 in liver,the mRNA expression levels of ZO-2,claudin-1 and closing protein(occludin)in jejunal mucosa at 28 d in PMLH group were all significantly increased(P<0.05 or P<0.01).It is concluded that dietary supplementation with PML can significantly improve growth performance,enhance the status of antioxidantion,immune and intestinal barrier function in broilers.
Perila mealyellow-feathered broilersgrowth performanceantioxidantionintestinal health
万方数据
维普
