摘要
本试验旨在研究紫苏粕(Perilla meal,PML)对肉鸡生长性能、血清生化、抗氧化功能及肠道组织形态的影响.选取1日龄健康黄羽肉鸡180羽,按照体质量随机分成4组,每组3个重复,每个重复15羽.对照组(CON组)饲喂基础日粮,试验组在基础日粮中分别添加0.25 g/kg(PMLL组)、0.50 g/kg(PMLM组)和1.00 g/kg(PMLH组)的紫苏粕,自由采食与饮水,试验期28 d.采集28日龄肉鸡血液、免疫器官和肠道组织,并测定抗氧化水平、免疫水平、肠道屏障基因表达水平及肠道绒毛高度(VH)、隐窝深度(CD)与绒隐比(V/C).结果表明,与CON组相比,PMLL组肉鸡平均体质量及空肠封闭蛋白-1(claudin-1)、闭锁小带蛋白-2(ZO-2)基因表达量均显著提高(P<0.05);PMLM组肉鸡平均体质量、空肠VH、V/C、闭锁小带蛋白-2(ZO-2)、黏蛋白-2(mucin-2)基因表达量以及肝脏磷酸化核因子2相关因子(Nrf-2)、醌氧化还原酶-1(NQO-1)和血红素加氧酶-1(HO-1)的基因表达量均显著提高(P<0.05),CD无显著性差异(P>0.05);PMLH组肉鸡血清SOD、GSH-Px活性和空肠闭合蛋白(occludin)、封闭蛋白-1(claudin-1)、闭锁小带蛋白-2(ZO-2)基因表达量以及肝脏Nrf-2、NQO-1和HO-1的基因表达量均显著提高(P<0.05或P<0.01).结果提示,日粮中添加PML可显著提高肉鸡生长性能,增强机体抗氧化功能,促进肠道绒毛发育.
Abstract
This experiment was conducted to investigate the effects of dietary Perila meal(PML)supplementation on growth performance,serum biochemical,antioxidantion and intestinal morphology of broilers.A total of 180 1-day-old yellow-feathered broilers were randomly assigned to 4 groups with 3 replicates per group and 15 birds per replicate.Broilers were fed a basal diet(control group),the basal diet supplemented with 0.25 g/kg(PMLL group),0.50 g/kg(PMLM group)or 1.00 g/kg(PMLH group)PML for 28 days.The broilers were provided the diets in ad libitum basis.Blood,immune organs and intestinal tissues were collected at 28 d,and the expression of genes,intestinal villus height and crypts depth were measured.The results showed as follows:1)compared with the CON group,the final weight,the mRNA expression levels of blocking protein-1(claudin-1)and blocking zonulin-2(ZO-2)in jejunal mucosa at 28 d(P<0.05)in PMLL group were all significantly increased(P<0.05);2)compared with the CON group,the final weight,the jejunal villus height,the ratio of jejunal villus height to crypt depth,the mRNA expression levels of mucin-2 and ZO-2 in jejunal mucosa,the mRNA expression levels of phosphorylated nuclear factor 2 related factor 2(Nrf-2),quinone oxidoreductase-1(NQO-1)and heme oxygenase-1(HO-1)in liver at 28 d in PMLM group were all increased significantly(P<0.05),but there was no significant difference in crypt depth between all groups(P>0.05);3)compared with the CON group,the levels of serum SOD and GSH-Px,the mRNA expression levels of Nrf-2,NQO-1 and HO-1 in liver,the mRNA expression levels of ZO-2,claudin-1 and closing protein(occludin)in jejunal mucosa at 28 d in PMLH group were all significantly increased(P<0.05 or P<0.01).It is concluded that dietary supplementation with PML can significantly improve growth performance,enhance the status of antioxidantion,immune and intestinal barrier function in broilers.
基金项目
海南省重点研发计划(ZDYF2023XDNY067)
维普
万方数据