In order to clarify the quality similarity of Radix Salviae Miltiorrhizae medicinal materials of different origin and production methods,explore the similarity of the main component characteristics and the similarity of the color difference fingerprint map,form a fast,scientific three-dimensional quality evaluation method,and use it for clinic and provide data reference for the production of medicinal materials in different regions.HPLC was used to establish the quality analysis method of the fingerprint characteristic graph of Radix Salviae Miltiorrhizae medicinal materials,through the Chinese medicine fingerprint map similarity evaluation system and the ModuleLab chroman software,the quality similarity evaluation and measurement analysis of the quality similarity of Radix Salviae Miltiorrhizae medicinal materials was carried out;the main component analysis(PCA),the most recently adjacent method(KNN)and other algorithms were used through the visualization method to analyze the overall distribution of the color value of the sample.The results showed that there were 11 peaks of Radix Salviae Miltiorrhizae medicinal materials in different places,and the similarity of the synthetic characteristic map was between 0.894-0.999,indicating that the sample chemical composition of different origin was small.The component PCA scoring load diagram and cluster analysis showed that the contribution rate of the main component was 85.93%.Among them,the ingredients of salvianolic acid B ingredients presented a high contribution rate to PC1,indicating that it was the main substance causing differences in different places.Samples of different habitats and the production method also had a large difference in total tanshinones.The total tanshinones content was 1.358-7.515 mg/g,and the content of salvianolic acid B was 4.574-112.670 mg/g.The color of the medicinal materials had extremely significant difference,especially the color difference in the color of the Radix Salviae Miltiorrhizae medicinal materials in Guangxi was more obvious,and the model recognition rate was 93.75%.This evaluation method can quickly distinguish the quality differences of medicinal materials,provide data reference for further clinical efficacy evaluation,and provide ideas and methods for the selection of Radix Salviae Miltiorrhizae medicinal materials and clinical applications.
关键词
丹参/药材/相似性评价/指纹图谱/计量分析/辨色论质
Key words
Radix Salviae Miltiorrhizae/medicinal material/similarity evaluation/fingerprint diagram/measurement analysis/color disciral theory
维普
万方数据