Composition difference of Fructus Toosendan-Fructus Foeniculi drug pair before and after salt roasting based on multivariate statistical analysis
To establish a HPLC fingerprint method for Fructus Toosendan-Fructus Foeniculi drug pair,and analyze and compare the differences in chemical composition of drug pair before and after salt roasting,providing reference for the study of its salt roasting mechanism and quality control.HPLC was used to establish the fingerprint spectra of the raw and salt roasted samples of the Fructus Toosendan and Fructus Foeniculi.The similarity evaluation system for traditional Chinese medicine fingerprint spectra(2012 version)was used to process the chromatograms.SMICA13.0 software was used to perform chemometric analysis on the common peak area data of 10 batches of raw and salt roasted samples.A total of 20 common peaks were calibrated,and 9 chromatographic peaks were identified through reference materials;through OPLS-DA analysis,a total of 14 common peaks were selected as the differential components(VIP>1)between the raw and salt roasted components of Fructus Toosendan and Fructus Foeniculi,after salt roasting,peaks of No.20(trans anethole),No.19(α-pinene),No.18(isotoosendanin),and No.15(toosendanin)decreased,while peaks of No.17,No.14,No.12,No.6(isoquercetin),No.13,No.16(kaempferol),No.5,and No.8 increased.Peaks of No.21(quercetin)and No.22 were newly added after salt roasting.The experimental results showed that there were significant differences in the components of Fructus Toosendan-Fructus Foeniculi drug pair before and after salt roasting,including toosendanin,isotoosendanin,trans anethole,α-pinene,kaempferol,quercetin and isoquercetin,which can serve as differential biomarkers for the drug pair.
Fructus Toosendan-Fructus Foeniculi drug pairsalt roastingfingerprintmultivariate statistical analysisdifference analysis