首页|基于多元统计分析的川楝子-小茴香药对盐炙前后化学成分差异研究

基于多元统计分析的川楝子-小茴香药对盐炙前后化学成分差异研究

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建立川楝子-小茴香药对的HPLC指纹图谱,分析比较川楝子-小茴香药对盐炙前后化学成分的差异,为其盐炙机理及质量控制研究提供参考.采用HPLC方法建立川楝子-小茴香药对生品和盐炙品的指纹图谱,采用"中药指纹图谱相似度评价系统(2012版)"对色谱图进行处理,采用SMICA13.0软件对生品和盐炙品各10批样品共有峰峰面积数据进行化学计量学分析.结果共标定了20个共有峰,通过对照品指认了其中9个色谱峰;通过OPLS-DA分析,共筛选出14个共有峰为川楝子-小茴香药对生品和盐炙品的差异性成分(VIP>1),分别为20号(反式茴香脑)、19号(α-蒎烯)、18号(川楝素)、15号(异川楝素)色谱峰(盐炙后响应降低),17号、14号、12号、6号(异槲皮素)、13号、16号(山奈酚)、5号、8号色谱峰(盐炙后响应增加),以及21号(槲皮素)、22号色谱峰(盐炙后新增加的色谱峰).试验结果表明川楝子-小茴香盐炙前后成分差异显著,川楝素、异川楝素、反式茴香脑、α-蒎烯、山奈酚、槲皮素、异槲皮素可作为二者的差异标志物.
Composition difference of Fructus Toosendan-Fructus Foeniculi drug pair before and after salt roasting based on multivariate statistical analysis
To establish a HPLC fingerprint method for Fructus Toosendan-Fructus Foeniculi drug pair,and analyze and compare the differences in chemical composition of drug pair before and after salt roasting,providing reference for the study of its salt roasting mechanism and quality control.HPLC was used to establish the fingerprint spectra of the raw and salt roasted samples of the Fructus Toosendan and Fructus Foeniculi.The similarity evaluation system for traditional Chinese medicine fingerprint spectra(2012 version)was used to process the chromatograms.SMICA13.0 software was used to perform chemometric analysis on the common peak area data of 10 batches of raw and salt roasted samples.A total of 20 common peaks were calibrated,and 9 chromatographic peaks were identified through reference materials;through OPLS-DA analysis,a total of 14 common peaks were selected as the differential components(VIP>1)between the raw and salt roasted components of Fructus Toosendan and Fructus Foeniculi,after salt roasting,peaks of No.20(trans anethole),No.19(α-pinene),No.18(isotoosendanin),and No.15(toosendanin)decreased,while peaks of No.17,No.14,No.12,No.6(isoquercetin),No.13,No.16(kaempferol),No.5,and No.8 increased.Peaks of No.21(quercetin)and No.22 were newly added after salt roasting.The experimental results showed that there were significant differences in the components of Fructus Toosendan-Fructus Foeniculi drug pair before and after salt roasting,including toosendanin,isotoosendanin,trans anethole,α-pinene,kaempferol,quercetin and isoquercetin,which can serve as differential biomarkers for the drug pair.

Fructus Toosendan-Fructus Foeniculi drug pairsalt roastingfingerprintmultivariate statistical analysisdifference analysis

王小鹏、马天翔、刘文海、侯建忠、朱顺娟

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甘肃省酒泉市中医院药剂科,甘肃酒泉 735008

甘肃省酒泉市药品检验检测中心中药检测科,甘肃酒泉 735008

川楝子-小茴香 盐炙 指纹图谱 多元统计分析 差异分析

甘肃省药品监督管理局科研项目甘肃省酒泉市科技计划

2022GSMPA00812023CA1014

2024

中兽医医药杂志
中国农业科学院中兽医研究所

中兽医医药杂志

影响因子:0.203
ISSN:1000-6354
年,卷(期):2024.43(2)
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