The objective was to simultaneous determine echinacoside and 2,3,5,4′-tetrahydroxy stilbene-2-Ο-β-D-glucoside in Congrong Tongbian oral liquid by HPLC.Tech Mate C18-ST(4.6 mm×250.0 mm,5 μm)column was used with gradient elution by acetonitrile-0.2%formic acid solution;the detection wavelength was 330 nm;the flow rate was 1.0 mL/min.The results showed that echinacoside and 2,3,5,4′-tetrahydroxy stilbene-2-Ο-β-D-glucoside in the sample presented good resolution from the adjacent chromatographic peaks with a good linear relationship in the range of 0.159 3-0.955 8 μg and 0.385 3-4.623 5 μg,respectively.The average recovery was 98.28%and 98.43%(RSD<2.0%).The content of two components in 10 batches of semples was 7.70-8.35 mg/mL and 8.80-14.70 mg/mL,respectively.The established method is simple and accurate,and can be used for the determination of echinacoside and stilbene glycoside in Congrong Tongbian oral liquid.
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