Simultaneous determination of seven constituents in Huixiang Juhe pills by HPLC
The purpose was to establish a HPLC method for the simultaneous determination of the main components of multiple medicinal herbs such as Pericarpium Citri Reticulatae Viride,Fructus Posraleae,Rhizoma Corydalis,Radix Aucklandiae,Fructus Foeniculi,Fructus Anisi Stellati,etc,in Huixiang Juhe pills.Huixiang Juhe pills was analyzed by Waters Symmetry C18 column(4.6 mm×250.0 mm,5 μm),with 0.02 mol/L potassium dihydrogen phosphate solution-acetonitrile-water as mobile phase,flowing at 1.0 mL/min in a gradient elution manner,detection wavelengths were set at 210 nm,290 nm and switch wavelengths in different time periods,column temperature was 31℃.As a result,hesperidin,opsralen,tetrahydropalmatine,isopsoralen,costundide,trans anisole and dehydrocostus presented a good linear relationship with peak area within the concentration range of 60.42-362.55 μg/mL,4.88-29.25 μg/mL,40.72-244.35 μg/mL,5.20-31.20 μg/mL,10.72-64.35 μg/mL,6.28-37.65 μg/mL and 11.90-71.40 μg/mL,with R2 values of 0.999 7,0.999 3,0.999 7,1.000 0,0.999 9,0.999 8,and 0.999 7,respectively.The average recoveries were 90.33%-100.03%with the RSD of 1.26%-2.85%.The method is specific and accurate,which can provide reference for the content determination of Huixiang Juhe pills.
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