摘要
建立HPLC法同时测定茴香橘核丸中青皮、补骨脂、延胡索、木香、小茴香、八角茴香等多味药材主要成分含量的方法.采用Waters Symmetry C18(4.6 mm×250.0 mm,5 μm)色谱柱,以0.02 mol/L磷酸二氢钾溶液-乙腈-水为流动相,梯度洗脱,流速1.0 mL/min,检测波长为210 nm、290 nm(分时段切换),柱温31℃.结果显示,橙皮苷在60.42～362.55 μg/mL、补骨脂素在4.88～29.25 μg/mL、延胡索乙素在40.72～244.35 μg/mL、异补骨脂素在5.20～31.20 μg/mL、木香烃内酯在10.72～64.35 μg/mL、反式茴香脑在6.28～37.65 μg/mL、去氢木香内酯在11.90～71.40 μg/mL浓度范围内与峰面积的线性关系均良好,R2分别为0.9997、0.9993、0.9997、1.0000、0.9999、0.9998、0.9997,其平均加样回收率为90.33%～100.03%,RSD为1.26%～2.85%.该方法专属性强、测定结果准确,可为茴香橘核丸含量测定提供参考.
Abstract
The purpose was to establish a HPLC method for the simultaneous determination of the main components of multiple medicinal herbs such as Pericarpium Citri Reticulatae Viride,Fructus Posraleae,Rhizoma Corydalis,Radix Aucklandiae,Fructus Foeniculi,Fructus Anisi Stellati,etc,in Huixiang Juhe pills.Huixiang Juhe pills was analyzed by Waters Symmetry C18 column(4.6 mm×250.0 mm,5 μm),with 0.02 mol/L potassium dihydrogen phosphate solution-acetonitrile-water as mobile phase,flowing at 1.0 mL/min in a gradient elution manner,detection wavelengths were set at 210 nm,290 nm and switch wavelengths in different time periods,column temperature was 31℃.As a result,hesperidin,opsralen,tetrahydropalmatine,isopsoralen,costundide,trans anisole and dehydrocostus presented a good linear relationship with peak area within the concentration range of 60.42-362.55 μg/mL,4.88-29.25 μg/mL,40.72-244.35 μg/mL,5.20-31.20 μg/mL,10.72-64.35 μg/mL,6.28-37.65 μg/mL and 11.90-71.40 μg/mL,with R2 values of 0.999 7,0.999 3,0.999 7,1.000 0,0.999 9,0.999 8,and 0.999 7,respectively.The average recoveries were 90.33%-100.03%with the RSD of 1.26%-2.85%.The method is specific and accurate,which can provide reference for the content determination of Huixiang Juhe pills.
基金项目
甘肃省酒泉市科技计划(2023CA1014)
甘肃省药品监督管理局科研项目(2022GSMPA0081)
国家科技期刊平台
NSTL
万方数据