Exploration on the potential action mechanism of Flos Lonicerae in avian influenza in chickens based on network pharmacology and molecular docking
To explore the potential action mechanism of Flos Lonicerae in avian influenza in chickens through network pharmacology and molecular docking.The active ingredients and corresponding targets of Flos Lonicerae were obtained based on the Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform(TCMSP),and then the targets of the diseases were searched and integrated by GeneCard,OMIM and TTD databases,and the intersecting targets of Flos Lonicerae and chicken avian influenza were analyzed using the STRING database,and the PPI network diagram was established.Core targets in the PPI network were screened in Cytoscape 10.1.1 software,and these targets were analyzed for GO function annotation and KEGG pathway enrichment in the DAVID database.The results showed that the main active components of Flos Lonicerae were screened as beta-carotene,luteolin,quercetin,and kaempferol,and there were 33 common targets with the disease,and the PPI network showed that the core targets were B lymphoblastoma-2 gene(BCL-2),albumin(ALB),MYC transcription factor(MYC),cystatinase 3(CASP-3),interleukin-6(IL-6)and transforming growth factor β1(TGF-β1),among others.GO functional annotation analysis resulted in 28 entries for biological processes,3 entries for cellular components,and 6 entries for molecular functions(P<0.05).KEGG pathway enrichment analysis yielded a total of 15 signaling pathways associated with avian influenza in chickens(P<0.05).The results of molecular docking showed that luteolin and BCL-2,ALB as well as beta-carotene and IL-6 exhibited good binding.The results indicated that the main active components of Flos Lonicerae,beta-carotene,luteolin,quercetin,and kaempferol,might play a role through the core targets of BCL-2,ALB,MYC,CASP-3,IL-6,and TGF-β1,as well as the related TLRs signaling pathway,MAPK signaling pathway,CLRs signaling pathway,and FoxO signaling pathway,playing an important role in the prevention of avian influenza in chickens.
国家科技期刊平台
NSTL
万方数据
