Residual detection of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline in hybutimibe by HPLC-MS/MS
A high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-MS/MS)method was established to determinate the residues of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline in hybutimibe.The compounds were separated by high performance liquid chromatography with Waters ACQUITY UPLC® BEH C18(2.1 mm×100.0 mm,1.7 μm)column.0.1%formic acid solution was used as the mobile phase A and methanol as the mobile phase B in gradient elution.The detector was triple quadrupole mass spectrometry.The MS data for the compounds were collected by positive ion electrospray ionization(ESI)using multi reaction monitoring mode(MRM).The linear ranges of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline were 1.000 0-20.000 0 ng/L,correlation coefficients(r)were 0.997 5-0.999 9.The detection limit concentrations were 0.33 mg/kg,and the quantification limit concentrations were 1.00 mg/kg.The method recovery rates were 85%-95%.The method is simple with good linear relationship,low detection limit and high recovery rate,which provide a technical support for the quality control of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline in hybutimibe.
high performance liquid chromatographytriple quadrupole mass spectrometry4-aminotoluene4-fluoroaniline2-fluoroanilineresidual research
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