In order to develop RT-PCR method for detection of Apple mosaic virus (ApMV) in field and make clear the occurrence of ApMV in China, RT-PCR detection system, including RNA-extraction method, reaction system and program of PCR, was optimized using the apple tissue infected by ApMV. The occurrence of ApMV in 13 provinces (23 cities/counties) of China was clarified by the modified RT- PCR assay. The result showed that the sensitivity of the optimized RT-PCR method could reach as less as 15 Ixg fresh sample using the total RNA extracted by the improved RNA-extraction method as template. In addition, the modified RT-PCR assay could detect ApMV using the bark tissue of one-year old branch as material during the whole growth period. In China, ApMV was detected in all of the 23 cities (coun- ties). The ApMV positive rate of the 327 samples reached 80.1% and the positive rate of the 205 symp- tomless samples was 68.3%. The positive rate was relative lower in three areas, including Zhangjiakou City of Hebei, Sanmenxia City of Henan and Baishui City of Shaanxi, which was lower than 50%.
Apple mosaic virusRNA extractionRT-PCRdistribution
NETL
NSTL
万方数据
维普
