首页|三种杀菌剂对牡丹黑斑病菌菌丝生长及分生孢子萌发的影响

三种杀菌剂对牡丹黑斑病菌菌丝生长及分生孢子萌发的影响

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为筛选防治牡丹黑斑病的化学药剂,采用菌丝生长速率法和涂布平板法分别测定了多菌灵、苯醚甲环唑和嘧菌酯对牡丹黑斑病菌菌丝生长的抑制活性及对分生孢子萌发和形成的影响.结果表明:苯醚甲环唑对菌丝生长的抑制作用最强,有效中浓度EC50仅为0.30μg/mL,其次为嘧菌酯6.34 μg/mL和多菌灵92.18μg/mL;多菌灵可显著降低分生孢子的萌发速率,而苯醚甲环唑和嘧菌酯对孢子萌发的抑制作用较弱,可使分生孢子及芽管发生畸形,并改变分生孢子的萌发方式;3种药剂均可显著抑制芽管的伸长生长,推迟病菌分生孢子梗的形成时间,但对分生孢子的产生数量无显著影响.多菌灵可作为保护剂在病害发生前期使用,苯醚甲环唑和嘧菌酯可在病害发生期轮换使用.
Effects of carbendazim, difenoconazole and azoxystrobin on mycelial growth and conidial germination of Alternaria suffruticosae
In order to screen fungicides to control peony black spot caused by Alternaria suffruticosae,the inhibitory activity of carbendazim,difenoconazole and azoxystrobin against mycelial growth of A.suffruticosae was measured and the efficacy of the three fungicides to the conidial germination and sporulation was determined.The results showed that difenoconazole could strongly inhibit the mycelial growth with an ECs0 value of 0.30 μg/mL,followed by azoxystrobin (6.34 μg/mL) and carbendazim (92.18 μg/mL).Carbendazim decreased conidial germination rate,whereas the effects of difenoconazole and azoxystrobin were inferior.Difenoconazole and azoxystrobin could cause abnormality of conidia and the germ tubes,affecting conidial germination by increasing the germinated tubes from the middle parts of conidia.All of the fungicides could strongly inhibit the germ tube growth of the conidia,delay sporulation,but have little effect on the conidial yields.Carbendazim could be used as a protective fungicide before the appearance of the peony black spot; difenoconazole could be used as a curative fungicide when the disease has been prevalent,and alternate use of difenoconazole and azoxystrobin might delay the onset of resistance.

peony black spotfungicidemycelial growthconidial germinationabnormality

侯颖、徐建强、宋宇州、胡建功、都胜芳、康业斌

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河南科技大学食品与生物工程学院,洛阳471023

河南科技大学林学院,洛阳471003

牡丹黑斑病 杀菌剂 菌丝生长 孢子萌发 致畸作用

公益性行业(农业)科研专项

201303023

2014

植物保护学报
中国植物保护学会 中国农业大学

植物保护学报

CSTPCDCSCD北大核心
影响因子:1.207
ISSN:0577-7518
年,卷(期):2014.41(3)
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