Effects of carbendazim, difenoconazole and azoxystrobin on mycelial growth and conidial germination of Alternaria suffruticosae
In order to screen fungicides to control peony black spot caused by Alternaria suffruticosae,the inhibitory activity of carbendazim,difenoconazole and azoxystrobin against mycelial growth of A.suffruticosae was measured and the efficacy of the three fungicides to the conidial germination and sporulation was determined.The results showed that difenoconazole could strongly inhibit the mycelial growth with an ECs0 value of 0.30 μg/mL,followed by azoxystrobin (6.34 μg/mL) and carbendazim (92.18 μg/mL).Carbendazim decreased conidial germination rate,whereas the effects of difenoconazole and azoxystrobin were inferior.Difenoconazole and azoxystrobin could cause abnormality of conidia and the germ tubes,affecting conidial germination by increasing the germinated tubes from the middle parts of conidia.All of the fungicides could strongly inhibit the germ tube growth of the conidia,delay sporulation,but have little effect on the conidial yields.Carbendazim could be used as a protective fungicide before the appearance of the peony black spot; difenoconazole could be used as a curative fungicide when the disease has been prevalent,and alternate use of difenoconazole and azoxystrobin might delay the onset of resistance.
peony black spotfungicidemycelial growthconidial germinationabnormality