Effects of several factors on the production of pycnidia and conidia in asparagus stem blight pathogen
To verify the optimized spore-production conditions of asparagus stem blight pathogen,the effects of different factors on pycnidium and conidium production were studied by using the petri-dish culture method with different media,temperature,illumination,and carbon and nitrogen sources.The results showed that the amounts of pycnidia and conidia in asparagus stem blight fungus were more on the nutrient-rich media,i.e.PDA,NLPDA and OLPDA,with over 10 per dish and 2.0 × 107 per dish,respectively,while the amounts of pycnidia and conidia on WA and Czapec plates were low.The pycnidium and conidium amounts of asparagus stem blight fungus at 28℃ were the largest with more than 10 per dish and 5.0 × 107 per dish,respectively.When the temperature went up to 34℃,almost no pycnidium and conidium were produced.The pycnidium and conidium amounts of asparagus stem blight fungus under 24 h darkness were largest with over 40 per dish and 5.0 × 107per dish,respectively.The amount was the least at full dark.All carbon sources could not improve the pycnidium and conidium productions obviously,and the amounts were almost 0.When yeast extract was the nitrogen source,both of the pycnidium and conidium productions were high.When ammonium sulfate as the nitrogen source,their production were almost 0.The results indicated that culture,temperature,and illumination affected the spore production,and few nitrogen source could improve the production,while carbon source almost had no effect.
NETL
NSTL
万方数据
维普
