Effects of Nilaparvata lugens (St(a)l) infestation on the trehalose contents and trehalase activities in both rice and brown planthoppers
To clarify the interaction between different rice varieties and Nilaparvata lugens (St(a)tl),TN1 (susceptible variety),Zhongzheyou No.1 (major variety in Zhejiang) and IR56 (insect-resistant variety) were chosen to determine the effects of N.lugens infestation on the trehalose contents and trehalase activities in both rice and N.lugens by using anthrone-sulfuric acid method and glucose analytical reagent,respectively.The results showed that the trehalase activity was increased from 3.56 and 4.34 nmol· g-1 · min-1 to 7.53 and 6.05 nmol· g-1· min-1 in IR56 and Zhongzheyou No.1 infested by N.lugens,and the trehalose content was decreased from 12.10 and 5.14 nmol/pg to 3.14 and 2.43nmol/μg.While the trehalase activity was increased from 1.46 nmol/μg to 3.05 nmol/μg in TN1 group,the trehalose content was not significantly changed.The trehalose contents in N.lugens feeding on TN1,IR56 and Zhongzheyou No.1 were 0.53,0.28 and 0.20 nmol/μg,respectively.Compared with TN1group,the trehalose content in IR56 and Zhongzheyou No.1 groups were significantly lower,and the trehalase activity and expression of three trehalase genes at mRNA expressional level were also declined significantly.These results indicated that trehalose and trehalase play important roles in the interaction between insect-resistant rice and N.Lugens.
NETL
NSTL
万方数据
维普
