Investigation on screening fungicide technique in vivo for controlling bacterial soft rot of vegetables
In order to explore a swift,efficient screening method to evaluate bactericide activities against bacterial soft rot of vegetables which caused by Pectobacterium carotovorum,the experimental materials,ways of inoculation,bacteria concentrations were screened to establish screening technology.And then celery stem section method was used to evaluate the bactericide activities of 28 Bacillus subtilis wettable powders (WPs) against bacterial soft rot of vegetables.The results showed that the bactericide,inhibiting the pathogens that caused bacterial soft rot of vegetables,could be assessed efficiently with the method of celery stem section established in this study.The method was to dip celery stems in bacterium suspension for 20 min,dry naturally,soak in the bactericides for one hour,then investigat the incidence index after 36 h culture under 26-30℃ and RH 70％-85％.In addition,B.subtilis WP that IVF001,IVF002,IVF004,IVF015,IVF018,IVF021,IVF035 and IVF041 were quickly selected and with the control efficacy were 81.70％,94.77％,83.01％,92.16％,84.31％,96.08％,80.39％,89.55％,respectively.Moreover,five of the registered B.subtilis WP which controlling effect regression with celery stem section and potted method was significant relegated with r=0.878.In conclusion,celery stem section method was a successful screening method for discovering agrochemicals on bacterial soft rot of vegetables.
NETL
NSTL
万方数据
维普
