Cloning of the serine protease inhibitor from oriental armyworm Mythimna separata and analysis of its anti-Beauveria bassiana function
To investigate the function of serine protease inhibitor(Serpin)in the growth,development,and immune defense of M.separata,the cDNA sequences of MsSerpin-4 and MsSerpin-5 were cloned from oriental armyworm Mythimna separata,and real-time quantitative PCR(qRT-PCR)was used to determine the expression levels of MsSerpins in various tissues and developmental stages.The RNA in-terference approach was employed to examine the function of MsSerpins in the anti-B.bassiana pro-cess.MsSerpin-4 and MsSerpin-5(GenBank accession numbers:MZ577062 and MZ577063)had se-quence lengths of 1 938 bp and 2 618 bp,respectively,and open reading frames of 1 188 bp and 1 197 bp,encoding 395 and 398 amino acids,respectively.Both MsSerpins were expressed in different tissues and developmental stages.The first-instar larvae exhibited the lowest expression,while the adult stage showed the highest.Expression levels were the lowest in the salivary glands and highest in the midgut.The greatest inhibition of MsSerpins was achieved by RNA interference for six hours,resulting in 52.57％and 49.15％inhibition,respectively.Following MsSerpins inhibition,M.separata became more susceptible to B.bassiana,resulting in a 31.47％and 14.62％increase in corrected mortality,respective-ly.These findings suggest the involvement of two MsSerpins in the immunological response to B.bassi-ana infection,offering a new insight into the biological regulation of M.separata.
万方数据
维普
