Aphid endosymbiont Buchnera assists the aphid in inhibiting plant defenses
To investigate whether the aphid endosymbiont Buchnera assists aphids in inhibiting plant defenses,a mixture of antibiotics was used to remove Buchnera of cucurbit-specialized bio type of Aphis gossypii,assessed the fitness of antibiotic-treated aphids on cucumbers,as well as the defensive sub-stances and susceptibility of cucumbers infested by these antibiotic-treated aphids.The results revealed a 71.15％decrease in body weight and a 95.60％decrease in fecundity in the antibiotic-treated aphids compared to normal aphids,but their lifespan increased by 2.34 folds.The electrical penetration graph showed that the antibiotic-treated aphids produced nearly no E1 and E2 waveforms,reflecting phloem salivation and phloem sap ingestion,respectively.Furthermore,the antibiotic-treated aphids stimulated higher levels of H2O2 compared to untreated aphids.Over a 14-d infestation,the antibiotic-treated aphids exhibited a continuous increase in total jasmonic acid and a continuous decrease in total salicylic acid,while untreated aphids showed an initial rise in total jasmonic acid followed by a decrease and an initial decrease in total salicylic acid followed by an increase.The duration from contacting to stable feeding significantly prolonged,and fecundity in the first three days significantly decreased for aphids on cucumbers that were previously infested by antibiotic-treated aphids.These results indicate that,in addition to providing necessary nutrients for aphids,Buchnera also play a role in assisting aphids in sup-pressing host plant defenses.
万方数据
维普
