Cold storage for artificial rearing of diapause adults of multicolored Asian lady beetle Harmonia axyridis
To extend the shelflife of adults and improve the flexibility of large-scale production,the ef-fects of temperature,photoperiod,and cold storage parameters on diapause rate,intensity,and life-history traits of the multicolored Asian lady beetle,Harmonia axyridis were investigated.The results showed that diapause could be induced in beetles by low temperatures and short daylight,with a dia-pause rate exceeding 95.0％at 20 ℃ and 10 L:14 D photoperiod.Temperature had minimal effect on diapause duration;however,diapausing H.axyridis at 15 ℃ and 20 ℃ under a 10 L:14 D photoperiod could quickly terminate diapause when subjected to 25 ℃ and 14 L:10 D,with more than 90.0％of H.axyridis terminating diapause within 10 d.Compared to 8 ℃ and 12 ℃,the survival rate,total egg pro-duction,and feeding amount of H.axyridis significantly decreased at 4 ℃ after 30 d of cold storage.Cold storage duration significantly affected life-history traits of female H.axyridis adults,including feeding amount,longevity,pre-reproductive period,total egg production,and hatching rate,while it had no significant effect on feeding amount of male adults.Lipids and sugars were identified as the main energy-consuming substances during cold storage of H.axyridis,and survival rate was positively corre-lated with total lipid and carbohydrate content during cold storage.In summary,in mass production,the optimal conditions for diapause induction in H.axyridis were found to be 20 ℃,10 L:14 D photoperiod.Furthermore,diapausing beetles stored at 12 ℃ for less than 90 d did not reduce their biological fitness.
万方数据
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