Host adaptation of Asian longhorn beetle Anoplophora glabripennis to Salix species in Ningxia and Zhejiang,China
To investigate the difference in the damages of Salix babylonica ×S.alba-vitellina and S.mat-sudana caused by Asian longhorn beetle Anoplophora glabripennis in Ningxia Hui Autonomous Region and Zhejiang Province,the differences in the secondary metabolites of the xylem of the two willow trees were analyzed by metabolomics.16S rDNA sequencing technology was used to detect the intesti-nal bacterial community composition of A.glabripennis larvae from two regions after feeding on S.bab-ylonica×S.alba-vitellina or S.matsudana.The activities of intestinal digestive enzymes,detoxification enzymes and protective enzymes were also measured.Spearman correlation analysis was carried out be-tween the secondary substances of two Salix species and intestinal bacteria and enzyme activity of A.glabripennis.The results showed that a total of 26 insect-resistant differential metabolites were screened from the xylem of S.babylonica×S.alba-vitellina and S.matsudana.Among them,the contents of ben-zoic acid and its derivatives,cinnamyl aldehyde,indole and its derivatives,and cinnamyl aldehyde were higher in S.matsudana.In contrast,the expressions of flavonoids,stilbene,ethyleneurea,and diazine were higher in S.babylonica×S.alba-vitellina.The dominant larval intestinal bacterial genera on S.mat-sudana were Enterococcus,Raoultella,unidentified Enterobacteriaceae,Pseudomonas,Enterobacter and Cellulomonas.Raoultella and Gibbsiella were the dominant larval intestinal bacteria of A.gla-bripennis feeding on S.babylonica×S.alba-vitellina.The activities of exo-β-1,4-glucanase,lipase,car-boxylesterase,glutathione-S-transferase and cytochrome P450 activities in the intestinal samples of lar-vae feeding on S.matsudana were significantly higher than those on S.babylonica ×S.alba-vitellina.The content of flavonoid in S.babylonica ×S.albavitellina was high with a substantial variable impor-tance in the projection value.It was speculated that the dominant bacterial species from the genus of Raoultella and Gibberella in the gut of A.glabripennis could easily metabolize flavonoids,leading to predominantly inactivated detoxification enzymes,which was accompanied by significant quantities of the bacterial community of Raoultella and Gibberella,although the levels of detoxification enzyme ac-tivity in the larval intestine were higher likely due to the absence of bacterial populations that can effi-ciently metabolize substances such as benzoic acid and its derivatives and cinnamyl aldehyde in the gut of A.glabripennis.The results indicated that,during the adaptation process of A.glabripennis to its host plant,the bacteria in its gut play a crucial role in the metabolism of the host's secondary metabolites.
万方数据
维普
