Synergistic toxicity of Metarhizium anisopliae and FKBP52 against the western flower thrips Frankliniella occidentalis
To elucidate the synergistic mechanism of Metarhizium anisopliae and insect immunosup-pressive proteins,the toxicity of the immunosuppressive protein FKBP52 and M.anisopliae to western flower thrips Frankliniella occidentalis were evaluated.The activities of carboxylesterase(CarE),glutathione-S transferase(GST),phenoloxidase(PO),and chitinase in F intonsa also were assessed us-ing the impregnation method.The results revealed that the median lethal time(LT50)for the combination of M.anisopliae and FKBP52 protein against F.intonsa was 7.32 d,demonstrating enhanced effective-ness compared to M.anisopliae(10.87 d)or FKBP52 protein(14.15 d)alone.When combined for nine days,the mortality rate of F.intonsa reached 81.11％,significantly higher than the rates observed with M.anisopliae(45.56％)or FKBP52 protein(36.67％)alone.The synergistic index of this mixture was 24.10,indicating a notable synergistic effect.Compared to the use of M.anisopliae alone,the activity levels of CarE and PO in F.intonsa initially decreased and then increased from day 1 to day 3 following the combined application of M.anisopliae and FKBP52 protein.The GST activity was significantly el-evated only on day 2.Chitinase activity decreased on day 2 and then increased on day 3.These findings suggest that the time-dependent modulation of CarE,GST,PO,and chitinase activities in F intonsa fol-lowing the combined application of M.anisopliae and FKBP52 protein could be a crucial mechanism underpinning their synergistic effect.
万方数据
维普
