Generation of PVX resistant potato plants by CRISPR/Cas13a editing technology
To investigate the feasibility of utilizing the CRISPR/Cas13a system to confer resistance against potato virus X(PVX)in Solanum tuberosum,a targeted small guide RNA(sgRNA)was de-signed to disrupt the TGBp1 gene of PVX and was employed in constructing a CRISPR/Cas13a gene-editing vector.The potato cultivar Désirée was chosen for stable genetic transformation.The results showed that transgenic potato lines expressing Cas13a were successfully developed and subsequently subjected to assessment for PVX resistance via mechanical inoculation.Among the transgenic lines,three demonstrated robust Cas13a expression and exhibited resistance to PVX.These resistant lines dis-played an absence of disease symptoms on systemically infected leaves and showcased a substantial re-duction in PVX accumulation between 10 to 20 days post-inoculation.These findings underscore the po-tency of the CRISPR/Cas13a system in effectively targeting PVX,thereby impeding virus accumula-tion.This study delineated the promising potential of harnessing CRISPR/Cas13a as a viable strategy for breeding PVX-resistant potatoes.
万方数据
维普
