Functional analysis of autophagy-related genes SsATG5 and SsATG8 in pathogenic fungus Sclerotinia sclerotiorum
In order to explore the functions of autophagy in the pathogenesis of Sclerotinia sclerotio-rum,the putative autophagy-related gene(ATG)was identified by homologous alignment of protein se-quences of yeast autophagy-related genes.According to the principle of homologous recombination,the ATG was knocked out and then complemented in the S.sclerotiorum strain 1980,and then the pheno-type of growth and virulence of different mutants were determined.The results showed that SsATG5 and SsATG8 were identified in S.sclerotiorum,and the expression of both of them were significantly up-regulated during the infection of S.sclerotiorum.There was no significant difference between SsATG5 and SsATG8 knockout mutants and wild-type strain 1980 in mycelial growth,oxalic acid production and infection cushion formation.However,the virulence of SsATG5 knockout mutants significantly de-creased by about 40％on the detached leaves of A.thaliana,while it significantly decreased by about 80％on A.thaliana plants.Importantly,the virulence of SsATG5 complementary strain was not different from that of the wild-type strain 1980.The results indicated that SsATG5 was involved in the pathogene-sis of S.sclerotiorum,and suggested that the autophagy pathway played important roles in the pathogen-esis of S.sclerotiorum.
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