Screening,identification and biocontrol efficacy of antagonistic bacterial strain against wheat stripe rust
In order to obtain bacterial strains with biocontrol potential against wheat stripe rust,microbi-al strains were isolated with soil dilution method,and those have antagonistic effects against Puccinia striiformis f.sp.tritici(Pst)were screened through assessment of antagonistic activity in Petri dishes.Morphological,physiological,and biochemical analyses,as well as 16S rDNA phylogenetic analysis,were conducted on strains exhibiting strong antagonistic effects.Their antifungal activity,antifungal spectrum,and biocontrol properties were assessed,with further validation through pot experiments.The results showed that strain XH effectively inhibited Pst urediniospore germination.Based on morphologi-cal and molecular characteristics,strain XH was identified as Streptomyces rectiviolaceus.The germina-tion inhibition rate of the fermentation liquid without Streptomyces cell of strain XH on Pst uredinio-spores was 94.36％,with a lethality rate of 91.53％.Additionally,this strain demonstrates inhibitory ef-fects on seven common plant pathogens and has the ability to secrete amylase,protease,cellulase,and siderophore,along with nitrogen fixation capabilities.The pot experiment results indicated that treat-ment with the fermentation liquid without Streptomyces cell and Streptomyces cell suspension of strain XH significantly reduced Pst infection on wheat leaves,resulting in control efficacies of 67.22％and 54.26％,respectively,for wheat stripe rust.These results indicated that the strain XH had potential for development and application as a biocontrol strain against the wheat stripe rust.
wheat stripe rustidentificationStreptomyces rectiviolaceusantimicrobial activitybiolog-ical control
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