小麦条锈病生防菌株的筛选、鉴定及其防治效果
Screening,identification and biocontrol efficacy of antagonistic bacterial strain against wheat stripe rust
肖珂雨 1贾瑞敏 1杨利飒 1马青 1王阳1
作者信息
- 1. 西北农林科技大学植物保护学院,陕西杨凌 712100
- 折叠
摘要
为获得对小麦条锈病具有生防潜力的菌株,通过土壤稀释法和皿内拮抗法分离筛选对小麦条锈菌Puccinia striiformis f.sp.tritici(Pst)有良好拮抗作用的菌株,基于形态学观察、生理生化特性和16S rDNA系统发育分析对其进行分类鉴定,测定其抑菌活性、抑菌谱及生防特性,并通过盆栽试验进一步验证其生防潜力.结果显示,筛选出1株菌株XH可以显著抑制Pst夏孢子萌发,结合形态学和分子生物学特征将其鉴定为直丝紫链霉菌Streptomyces rectiviolaceus;菌株XH发酵滤液对Pst夏孢子的萌发抑制率为94.36％,致死率达91.53％,且该菌株对测试的7种常见植物病原菌均有抑制作用,可以分泌淀粉酶、蛋白酶、纤维素酶和嗜铁素,具有固氮作用;盆栽试验结果显示,菌株XH的菌体悬浮液和发酵滤液可显著降低小麦叶片的Pst侵染量,对小麦条锈病的防治效果分别为54.26％和67.22％.表明菌株XH作为小麦条锈病生防菌株具有较好的开发应用潜力.
Abstract
In order to obtain bacterial strains with biocontrol potential against wheat stripe rust,microbi-al strains were isolated with soil dilution method,and those have antagonistic effects against Puccinia striiformis f.sp.tritici(Pst)were screened through assessment of antagonistic activity in Petri dishes.Morphological,physiological,and biochemical analyses,as well as 16S rDNA phylogenetic analysis,were conducted on strains exhibiting strong antagonistic effects.Their antifungal activity,antifungal spectrum,and biocontrol properties were assessed,with further validation through pot experiments.The results showed that strain XH effectively inhibited Pst urediniospore germination.Based on morphologi-cal and molecular characteristics,strain XH was identified as Streptomyces rectiviolaceus.The germina-tion inhibition rate of the fermentation liquid without Streptomyces cell of strain XH on Pst uredinio-spores was 94.36％,with a lethality rate of 91.53％.Additionally,this strain demonstrates inhibitory ef-fects on seven common plant pathogens and has the ability to secrete amylase,protease,cellulase,and siderophore,along with nitrogen fixation capabilities.The pot experiment results indicated that treat-ment with the fermentation liquid without Streptomyces cell and Streptomyces cell suspension of strain XH significantly reduced Pst infection on wheat leaves,resulting in control efficacies of 67.22％and 54.26％,respectively,for wheat stripe rust.These results indicated that the strain XH had potential for development and application as a biocontrol strain against the wheat stripe rust.
关键词
小麦条锈病/鉴定/直丝紫链霉菌/抗菌活性/生物防治Key words
wheat stripe rust/identification/Streptomyces rectiviolaceus/antimicrobial activity/biolog-ical control引用本文复制引用
基金项目
国家重点研发计划(2021YFD140100502)
出版年
2024
NETL
NSTL
万方数据