Glycoside hydrolase gene FvGH16-2 is involved in the growth and pathogenicity of mycotoxigenic fungus Fusarium verticillioides
To clarify the role of glycoside hydrolase 16(GH16)family genes in the growth,develop-ment,and pathogenesis of mycotoxigenic fungus Fusarium verticillioides,bioinformatics software was used to identify GH16 family members,and transcriptome and real-time fluorescence quantitative PCR techniques were used to analyze the expression levels of GH16 family members at different culture tem-peratures.The FvGH16-2 gene was knocked out and replaced by genetic transformation to analyze its role in growth,development,and pathogenesis of F.verticillioides.The results showed that a total of 23 GH16 family genes were identified in F verticillioides genome,among which FvCH16-2,FvCH16-4,FvCH16-12 and other up-regulated genes were associated with the growth and stress resistance of patho-gens.FvGH16-2 gene knockout resulted in decreased growth rate and conidial production of F verticil-lioides,impaired cell wall integrity,and increased sensitivity to H2O2.In addition,FvGH16-2 gene knockout reduced the pathogenicity of F verticillioides on maize seeds and maize stalks.These results indicated that the FvGH16-2 gene played an important role in the growth,development,and pathogene-sis of F.verticillioides,and was essential for the integrity of the cell wall and pathogenicity of F.verticil-lioides.
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