Effects of rice ragged stunt virus on the growth,development and reproduction of the brown planthopper Nilaparvata lugens
To clarify the effects of rice ragged stunt virus(RRSV)on the growth,development,and re-production of the brown planthopper Nilaparvata lugens,we conducted laboratory breeding experi-ments to assess various growth and life table parameters,including larval duration,adult longevity,egg production,sex ratio,and brachypterous ratio of N.lugens.Additionally,we measured the expression lev-els of vitellogenin(Vg),vitellogenin receptor(VgR),and genes involved in the trehalose metabolic pro-cess using real-time quantitative PCR(RT-qPCR)between viruliferous and non-viruliferous N.lugens.The results showed that the nymphal duration of viruliferous N.lugens was significantly prolonged com-pared to non-viruliferous N.lugens,with durations of 17.56 d and 15.90 d,respectively.Moreover,virulif-erous N.lugens displayed slightly higher adult longevity,female ratio,and brachypterous ratio compared to their non-viruliferous counterparts.The fecundity of viruliferous N.lugens was significantly elevated,with an average of 11.26 eggs per female compared to 6.45 eggs per female for non-viruliferous individuals.Furthermore,significantly higher expression levels of Vg,VgR,and the trehalose transport-er gene(TRET)were observed in viruliferous N.lugens compared to non-viruliferous individuals.These results indicated that RRSV invasion could significantly prolong the nymphal duration and increase N.lugens fecundity,potentially attributed to the up-regulated expression levels of Vg,VgR,and TRET.
rice ragged stunt virusNilaparvata lugensgrowth and developmentfecundity
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