摘要
为明确水稻锯齿叶矮缩病毒(rice ragged stunt virus,RRSV)对褐飞虱Nilaparvata lugens生长发育和生殖的影响,分别比较带毒褐飞虱与未带毒褐飞虱的若虫发育历期、成虫寿命、性比、翅型及产卵量,并利用实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)技术分析RRSV侵染后褐飞虱体内卵黄原蛋白(vitellogenin,Vg)与其受体(vitellogenin receptor,VgR)以及海藻糖代谢途径中相关基因表达量的情况.结果显示,带毒褐飞虱的若虫历期显著高于未带毒褐飞虱的若虫历期,分别为17.56 d和15.90 d;带毒褐飞虱的成虫寿命、雌虫比例和短翅比例均高于未带毒褐飞虱的,但两者间均无显著差异;带毒褐飞虱较未带毒褐飞虱单雌日产卵量显著提升,分别为11.26粒和6.45粒;带毒褐飞虱的Vg、VgR和海藻糖转运蛋白基因(trehalose transporter,TRET)表达量均显著高于未带毒褐飞虱的.表明RRSV侵染会显著延长褐飞虱若虫发育历期,同时可能通过上调Vg、VgR以及TRET的表达量以促进褐飞虱生殖.
Abstract
To clarify the effects of rice ragged stunt virus(RRSV)on the growth,development,and re-production of the brown planthopper Nilaparvata lugens,we conducted laboratory breeding experi-ments to assess various growth and life table parameters,including larval duration,adult longevity,egg production,sex ratio,and brachypterous ratio of N.lugens.Additionally,we measured the expression lev-els of vitellogenin(Vg),vitellogenin receptor(VgR),and genes involved in the trehalose metabolic pro-cess using real-time quantitative PCR(RT-qPCR)between viruliferous and non-viruliferous N.lugens.The results showed that the nymphal duration of viruliferous N.lugens was significantly prolonged com-pared to non-viruliferous N.lugens,with durations of 17.56 d and 15.90 d,respectively.Moreover,virulif-erous N.lugens displayed slightly higher adult longevity,female ratio,and brachypterous ratio compared to their non-viruliferous counterparts.The fecundity of viruliferous N.lugens was significantly elevated,with an average of 11.26 eggs per female compared to 6.45 eggs per female for non-viruliferous individuals.Furthermore,significantly higher expression levels of Vg,VgR,and the trehalose transport-er gene(TRET)were observed in viruliferous N.lugens compared to non-viruliferous individuals.These results indicated that RRSV invasion could significantly prolong the nymphal duration and increase N.lugens fecundity,potentially attributed to the up-regulated expression levels of Vg,VgR,and TRET.
基金项目
福建省重大专项(2022NZ030014)
福建省大学生创新创业训练计划(S202310389067)
福建农林大学创新基金(KFb22014XA)
福建省自然科学基金(2020J01596)
NETL
NSTL
万方数据