Knocking out OsLRR-RLK18 in rice enhances the resistance to brown planthopper Nilaparvata lugens
To elucidate the role of the leucine-rich repeat receptor-like kinase(LRR-RLK)gene OsLRR-RLK18 in regulating rice defense responses against the brown planthopper Nilaparvata lugens and its underlying mechanisms,we utilized both the wild-type rice line and OsLRR-RLK18 knockout rice lines as research materials to assess the shoot height and root length of different rice plants,examine the ef-fect on N.lugens biological parameters across different rice lines,and analyze the levels of defense-related signal molecules and defensive compounds in different rice plants before and after infestation by N.lugens females.The results showed that knocking out OsLRR-RLK18 significantly increased the lev-els of jasmonic acid,jasmonic acid-isoleucine,and abscisic acid in rice at late stage of N.lugens infesta-tion,but decreased the basal(non-infested)and N.lugens-induced(earlier stage of infestation)levels of salicylic acid and hydrogen peroxide.Moreover,knocking out OsLRR-RLK18 enhanced the emission of multiple volatile compounds from rice plants while reducing the levels of lignin,p-coumaroyl putres-cine,and three flavonoids(carlinoside,cosmosiin and prunin).These changes finally led to a significant decrease in the number and hatchability of N.lugens eggs.These results indicate that OsLRR-RLK18 is involved in the defense responses in rice induced by N.lugens infestation,and plays a role in regulating rice the resistance to N.lugens.
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