Safety evaluation of Cry2Aa protein on Cotesia chilonis using feeding and micro-injection methods
To evaluate the safety of transgenic Cry2Aa rice T2A-1 on the parasitoid wasp Cotesia chilo-nis,a comprehensive assessment was conducted using feeding and microinjection methods.The results revealed that no detectable Cry2Aa protein was found in parasitoid wasps in the rice stem borers Chilo suppressalis fed on transgenic Cry2Aa rice T2A-1.The number of cocoons,cocoon length,male adult lifespan,and sex ratio of C.chilonis fed on T2A-1 rice were significantly lower than those of the con-trol group,with values of 20.00,2.58 mm,2.34 d,and 3.10,respectively.However,the total developmen-tal duration for eggs and larvae,the pupal period,female adult lifespan,cocoon weight,and adult weight showed no significant differences from control group.In the case of microinjection of Cry2Aa protein into the rice stem borers,there were significant differences in most life table parameters(except the total developmental duration of egg and larva,and the pupal period),as well as the activities of per-oxidase,superoxide dismutase,and glutathione reductase enzymes compared to the positive control.However,there were no significant differences in all life table parameters and enzyme activities when compared to the negative control.These results indicated that the observed effects in the feeding method were mediated by the host-induced effects rather than the Cry2Aa protein itself,suggesting the safety of transgenic Cry2Aa rice T2A-1 on the parasitoid wasp C.chilonis.
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