Analysis of transcriptome and resistance-related genes induced by beta-cypermethrin in the grasshopper Oedaleus asiaticus(Orthoptera:Oedipodidae)
This study aimed to identify resistance-related genes and metabolic pathways in Oedaleus asiaticus in response to beta-cypermethrin stress and elucidate the resistance mechanism of O.asiaticus to beta-cypermethrin.The 3rd instar nymphs of O.asiaticus were treated with beta-cypermethrin(LD10=0.008 μg/nymph)for 48 h.The transcriptome sequencing was performed on pre-and post-processing samples by using the Illummina Hiseq 4000 high-throughput sequencing platform.Functional annota-tion,differential expression gene(DEG)analysis,and metabolic pathway analysis were performed us-ing bioinformatics tools BLAST,edgeR,and Trinity.The results revealed 743 DEGs in O.asiaticus af-ter treated with beta-cypermethrin,including 208 up-regulated genes and 535 down-regulated genes.Among them,168 DEGs were significantly enriched in three functional categories(cell components,molecular functions and biological processes)in the GO database,while 162 DEGs were significantly enriched in 20 different metabolic pathways,which were mainly involved in the metabolic pathways such as ribosome,carbon metabolism,glycolysis,and amino acid biosynthesis,with several metabolic pathways related to insecticide resistance.Further analysis identified 47 DEGs related to insect resis-tance,including glutathione S-transferase,alcohol dehydrogenase,cytochrome P450 gene family,heat shock protein,carboxylesterase,mucin,multifunctional oxidase,and carbonyl reductase.These genes play a role in the resistance of O.asiaticus to beta-cypermethrin stress.
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