Effect of host transformation on the survival rate and nutritional metabolism of cotton aphid,Aphis gossypii
To investigate the effect of host transformation on the survival rate and nutritional metabo-lism levels of cotton aphid,Aphis gossypii,the living plant feeding method was used to transfer cotton-type,wolfberry-type,and cucumber-type host-specialized cotton aphids to different hosts,and the sur-vival rates and changes in nutritional metabolism enzyme activity of cotton aphids after host conversion were determined.Compared to cotton aphids without host switching,the survival rates of cotton-type and cucumber-type cotton aphids significantly decreased after being transferred to wolfberry(with sur-vival rates of cotton-type and cucumber-type cotton aphids on wolfberry recorded at 61.5％and 62.1％,respectively).These survival rates were lower than those observed for cotton-type and cucumber-type cotton aphids that remained on their original hosts,which were 90.3％and 86.4％,respectively.Upon transfer to wolfberry at 96 h,the activities of amylase,trypsin,sucrase,and lipase in the bodies of cot-ton-type cotton aphids decreased to 58.3％,73.5％,90.4％and 83.7％of the control group for cotton aphids,65.1％,66.9％,and 85.3％of the control group for cucumber aphids,respectively.Conversely,the activity of trehalase increased to 2.2 times and 1.3 times of the control group for cotton and cucum-ber aphids,respectively.Notably,there were no significant differences in the survival rate and activity of nutritional metabolism enzymes of wolfberry-type cotton aphids when transferred to cotton or cucum-ber,compared to wolfberry-type cotton aphids that have not been transferred.In conclusion,transition-ing to different host plants induces significant changes in enzymatic activity related to nutritional metab-olism in different host-specialized cotton aphids.Particularly,a disruption in metabolic enzyme activity and a decrease in survival rate were observed when cotton-type and cucumber-type cotton aphids were transferred to wolfberry.
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